Echinicola sediminis sp. Nov., a marine bacterium isolated from coastal sediment

A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2^T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2^T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were ‘Echinicola shivajiensis’ AK12^T (98.1% 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754^T (96.4 %), Echinicola jeungdonensis HMD 3054^T (96.0 %), Echinicola pacifica KMM 6172^T (96.0 %) and Echinicola rosea JL 3085^T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C_{15: 0}, summed feature 3 (comprising C_{16: 1}ω7c and/or C_{16: 1}ω6c), C_{17: 1}ω6c and summed feature 9 (comprising iso-C_{17: 1}ω9c and/or 10-methyl C_{16: 0}). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA– DNA relatedness between strain 001-Na2^T and ‘E. shivajiensis’ JCM 17847^T was 20.8–21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA–DNA hybridization analyses presented, strain 001-Na2^T (=KCTC 52495^T=JCM 31786^T =DSM 103729^T) is the type strain of a novel species of the genus Echinicola, for which the name Echinicola sediminis sp. nov. is proposed.