Echinicola sediminis sp. Nov., a marine bacterium isolated from coastal sediment

Dong Wan Lee, Aslan Hwanhwi Lee, Hanbyul Lee, Jae Jin Kim, Jong Seong Khim, Un Hyuk Yim, Beom Seok Kim

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2T belonged to the genus Echinicola and these species formed a distinct lineage within the family Cyclobacteriaceae. The closest phylogenetic neighbours were ‘Echinicola shivajiensis’ AK12T (98.1% 16S rRNA gene sequence similarity), Echinicola vietnamensis LMG 23754T (96.4 %), Echinicola jeungdonensis HMD 3054T (96.0 %), Echinicola pacifica KMM 6172T (96.0 %) and Echinicola rosea JL 3085T (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C15: 0, summed feature 3 (comprising C16: 1ω7c and/or C16: 1ω6c), C17: 1ω6c and summed feature 9 (comprising iso-C17: 1ω9c and/or 10-methyl C16: 0). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA– DNA relatedness between strain 001-Na2T and ‘E. shivajiensis’ JCM 17847T was 20.8–21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA–DNA hybridization analyses presented, strain 001-Na2T (=KCTC 52495T=JCM 31786T =DSM 103729T) is the type strain of a novel species of the genus Echinicola, for which the name Echinicola sediminis sp. nov. is proposed.

Original languageEnglish
Article number002118
Pages (from-to)3351-3357
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume67
Issue number9
DOIs
Publication statusPublished - 2017 Sept

Bibliographical note

Funding Information:
This work was supported by the project entitled ‘Oil Spill Environmental Impact Assessment and Environmental Restoration (PM57431)’ funded by the Ministry of Oceans and Fisheries of Korea, the BK21 Plus program in 2013 (Project No. 21A20130012270) funded by the National Research Foundation of Korea (NRF) and a Korea University Grant.

Publisher Copyright:
© 2017 IUMS.

Keywords

  • Bacteroidetes
  • Coastal sediment
  • Echinicola
  • Marine bacterium

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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