Effect of a buffer mixture system on the activity of lipases during immobilization process

Jong Ho Lee; Sung Bong Kim; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.biortech.2009.03.031

Effect of a buffer mixture system on the activity of lipases during immobilization process

Jong Ho Lee, Sung Bong Kim, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

In this study, the effects of various buffers and ionic strengths on the immobilization of Candida rugosa and Rhizopus oryzae lipases were investigated to enhance the activities of the immobilized lipases. Among the various buffers, the optimal buffers and ionic strength for the immobilization of C. rugosa and R. oryzae lipases were determined to be a mixture of 0.25. M MOPs and sodium phosphate buffer (pH 6.5). Moreover, the activities of immobilized C. rugosa and R. oryzae lipases under their optimal conditions were 3756.11 and 2845.21. U/g matrix, respectively. Furthermore, the activity of immobilized lipases increased by approximately 4.13 and 3.1 times after 24. h, respectively. Finally, the activities of the immobilized lipases were maintained at levels greater than 90% of their original activities after ten reuses and at levels greater than 60% of their original activities after twenty reuses.

Original language	English
Pages (from-to)	S66-S70
Journal	Bioresource technology
Volume	101
Issue number	SUPPL.1
DOIs	https://doi.org/10.1016/j.biortech.2009.03.031
Publication status	Published - 2010 Jan

Bibliographical note

Funding Information:
This study was supported by the Brain Korea 21 program issued by the Ministry of Education, Korea.

Keywords

Buffer effect
Buffer mixture system
Ion strength
pH

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2009.03.031

Cite this

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title = "Effect of a buffer mixture system on the activity of lipases during immobilization process",

abstract = "In this study, the effects of various buffers and ionic strengths on the immobilization of Candida rugosa and Rhizopus oryzae lipases were investigated to enhance the activities of the immobilized lipases. Among the various buffers, the optimal buffers and ionic strength for the immobilization of C. rugosa and R. oryzae lipases were determined to be a mixture of 0.25. M MOPs and sodium phosphate buffer (pH 6.5). Moreover, the activities of immobilized C. rugosa and R. oryzae lipases under their optimal conditions were 3756.11 and 2845.21. U/g matrix, respectively. Furthermore, the activity of immobilized lipases increased by approximately 4.13 and 3.1 times after 24. h, respectively. Finally, the activities of the immobilized lipases were maintained at levels greater than 90% of their original activities after ten reuses and at levels greater than 60% of their original activities after twenty reuses.",

keywords = "Buffer effect, Buffer mixture system, Ion strength, pH",

author = "Lee, {Jong Ho} and Kim, {Sung Bong} and Chulhwan Park and Kim, {Seung Wook}",

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AU - Lee, Jong Ho

AU - Kim, Sung Bong

AU - Park, Chulhwan

AU - Kim, Seung Wook

N1 - Funding Information: This study was supported by the Brain Korea 21 program issued by the Ministry of Education, Korea.

PY - 2010/1

Y1 - 2010/1

N2 - In this study, the effects of various buffers and ionic strengths on the immobilization of Candida rugosa and Rhizopus oryzae lipases were investigated to enhance the activities of the immobilized lipases. Among the various buffers, the optimal buffers and ionic strength for the immobilization of C. rugosa and R. oryzae lipases were determined to be a mixture of 0.25. M MOPs and sodium phosphate buffer (pH 6.5). Moreover, the activities of immobilized C. rugosa and R. oryzae lipases under their optimal conditions were 3756.11 and 2845.21. U/g matrix, respectively. Furthermore, the activity of immobilized lipases increased by approximately 4.13 and 3.1 times after 24. h, respectively. Finally, the activities of the immobilized lipases were maintained at levels greater than 90% of their original activities after ten reuses and at levels greater than 60% of their original activities after twenty reuses.

AB - In this study, the effects of various buffers and ionic strengths on the immobilization of Candida rugosa and Rhizopus oryzae lipases were investigated to enhance the activities of the immobilized lipases. Among the various buffers, the optimal buffers and ionic strength for the immobilization of C. rugosa and R. oryzae lipases were determined to be a mixture of 0.25. M MOPs and sodium phosphate buffer (pH 6.5). Moreover, the activities of immobilized C. rugosa and R. oryzae lipases under their optimal conditions were 3756.11 and 2845.21. U/g matrix, respectively. Furthermore, the activity of immobilized lipases increased by approximately 4.13 and 3.1 times after 24. h, respectively. Finally, the activities of the immobilized lipases were maintained at levels greater than 90% of their original activities after ten reuses and at levels greater than 60% of their original activities after twenty reuses.

KW - Buffer effect

KW - Buffer mixture system

KW - Ion strength

KW - pH

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DO - 10.1016/j.biortech.2009.03.031

M3 - Article

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VL - 101

SP - S66-S70

JO - Bioresource technology

JF - Bioresource technology

IS - SUPPL.1

ER -

Effect of a buffer mixture system on the activity of lipases during immobilization process

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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