TY - JOUR
T1 - Effect of biodiesel-derived raw glycerol on 1,3-propanediol production by different microorganisms
AU - Moon, Chuloo
AU - Ahn, Jae Hyeong
AU - Kim, Seung W.
AU - Sang, Byoung In
AU - Um, Youngsoon
N1 - Funding Information:
Acknowledgments The authors would like to acknowledge the financial support of the Korea Ministry of Knowledge Economy (MKE) through the Energy Technology Innovation Project (ETI) and the Korean Research Foundation through the Korea-China Science and Technology Cooperation Center Program.
PY - 2010/5
Y1 - 2010/5
N2 - The microbial production of 1,3-propanediol (1,3-PD) from raw glycerol, a byproduct of biodiesel production, is economically and environmentally advantageous. Although direct use of raw glycerol without any pretreatment is desirable, previous studies have reported that this could cause inhibition of microbial growth. In this study, we investigated the effects of raw glycerol type, different microorganisms, and pretreatment of raw glycerol on the production of 1,3-PD. Raw glycerol from waste vegetable-oil-based biodiesel production generally caused more inhibition of 1,3-PD production and microbial growth compared to raw glycerol from soybean-oil-based biodiesel production. In addition, two raw glycerol types produced from two biodiesel manufacturers using waste vegetable oil exhibited different 1,3-PD production behavior, partially due to different amounts of methanol included in the raw glycerol from the two biodiesel manufacturers. Klebsiella strains were generally resistant to all types of raw glycerol while the growth of Clostridium strains was variably inhibited depending on the type of raw glycerol. The 1,3-PD production of the Clostridium strains using acid-pretreated raw glycerol was significantly enhanced compared to that with raw glycerol, demonstrating the feasibility of using raw glycerol for 1,3-PD production by various microorganisms.
AB - The microbial production of 1,3-propanediol (1,3-PD) from raw glycerol, a byproduct of biodiesel production, is economically and environmentally advantageous. Although direct use of raw glycerol without any pretreatment is desirable, previous studies have reported that this could cause inhibition of microbial growth. In this study, we investigated the effects of raw glycerol type, different microorganisms, and pretreatment of raw glycerol on the production of 1,3-PD. Raw glycerol from waste vegetable-oil-based biodiesel production generally caused more inhibition of 1,3-PD production and microbial growth compared to raw glycerol from soybean-oil-based biodiesel production. In addition, two raw glycerol types produced from two biodiesel manufacturers using waste vegetable oil exhibited different 1,3-PD production behavior, partially due to different amounts of methanol included in the raw glycerol from the two biodiesel manufacturers. Klebsiella strains were generally resistant to all types of raw glycerol while the growth of Clostridium strains was variably inhibited depending on the type of raw glycerol. The 1,3-PD production of the Clostridium strains using acid-pretreated raw glycerol was significantly enhanced compared to that with raw glycerol, demonstrating the feasibility of using raw glycerol for 1,3-PD production by various microorganisms.
KW - 1,3-Propanediol
KW - Fermentation
KW - Pretreatment
KW - Raw glycerol
UR - http://www.scopus.com/inward/record.url?scp=77949656495&partnerID=8YFLogxK
U2 - 10.1007/s12010-009-8859-6
DO - 10.1007/s12010-009-8859-6
M3 - Article
C2 - 19937397
AN - SCOPUS:77949656495
SN - 0273-2289
VL - 161
SP - 502
EP - 510
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 1-8
ER -