Effect of crude glycerol-derived inhibitors on ethanol production by Enterobacter aerogenes

Sang Jun Lee, Sung Bong Kim, Seong Woo Kang, Sung Ok Han, Chulhwan Park, Seung Wook Kim

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31 Citations (Scopus)


In this study, ethanol production from pure and crude glycerol using Enterobacter aerogenes ATCC 29007 was evaluated under anaerobic culture conditions. Inhibitory effects of substrate concentrations, pH, and salt concentrations were investigated based on crude glycerol components. Ethanol production was performed with pure glycerol concentrations ranging from 5 to 30 g/L to evaluate the effects of substrate concentration and osmotic pressure. The consumed glycerol was 5-14.33 g/L, and the yield of ethanol was higher than 0.75 mol ethanol/mol glycerol after 24 h of cultivation. To evaluate the inhibitory effects of salts (NaCl and KCl), experiments were performed with 0-20 g/L of each salt. Inhibitory effects of salts were strongest at high salt concentrations. The inhibitory effect of pH was performed in the pH range 4-10, and cell growth and ethanol production were highest at pH 5-6. Also, ethanol production was slightly inhibited at low concentration of crude glycerol comparison with pure glycerol. However, significant inhibitory effects were not observed at 1.5 and 2% crude glycerol which showed higher ethanol production compared to pure glycerol.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalBioprocess and Biosystems Engineering
Issue number1-2
Publication statusPublished - 2012 Jan

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Core Environmental Technology Development Project for Next Generation funded by the Ministry of Environment, Republic of Korea (No. 032-091-019). This research was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0005504) and Research Grant from Kwangwoon University (2012).


  • Enterobacter aerogenes
  • Ethanol production
  • Glycerol
  • Inhibitory effect

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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