Effect of denture cleansers on Candida albicans biofilm formation over resilient liners

Purpose: The purpose of this study was to analyze the effect of denture cleansers on Candida albicans biofilm formation over resilient liners and to evaluate compatibility between resilient liners and denture cleansers. Materials And Methods: Acrylic resin (Lucitone 199®) and 3 resilient liners (COE-SOFT™, GC RELINE™ and SOFRELINER TOUGH TOUGH®) were incubated in denture cleansers (Polident® and Cleadent®) for 8 hours a day and in unstimulated saliva for 16 hours a day (n=25/gp) for 60 days. Two-way and three-way repeated measures ANOVA were performed to compare the surface roughness (Ra), pH and C. albicans binding level by radioisotope (a=0.05). The statistical significance of the relation between Ra and adhesion was evaluated by correlation analysis. Results: The degree of Ra was significantly decreased in the following order: COE-SOFT™, acrylic resin, GC RELINE™ and SOFRELINER TOUGH®. The immersion in denture cleansers significantly increased Ra of resilient liners, except for SOFRELINER TOUGH® in Cleadent®. No significant differences in pH curves were observed among groups immersed in distilled water and denture cleansers. The binding levels of C. albicans were significantly decreased in the following order: COE-SOFT™, GC RELINE™, SOFRELINER TOUGH®, and acrylic resin. The immersion in Cleadent® seemed to decrease C. albicans binding level on GC RELINE™ and SOFRELINER TOUGH®. Conclusion: Based on the C. albicans binding levels results, it is not recommended to immerse COE-SOFT™ in denture cleansers, and GC RELINE™ and SOFRELINER TOUGH® should be immersed in Cleadent®.