Effect of genistein on the expression of bone metabolism genes in ovariectomized mice using a cDNA microarray

Jae Eun Pie; Jin Hee Park; Yoon Hee Park; Yeon Mi Ryu; Ki Nam Kim; Seung Woo Suh; Kevin G. Becker; Yoon S. Cho-Chung; Meyoung Kon Kim

doi:10.1016/j.jnutbio.2005.06.002

Effect of genistein on the expression of bone metabolism genes in ovariectomized mice using a cDNA microarray

Jae Eun Pie, Jin Hee Park, Yoon Hee Park, Yeon Mi Ryu, Ki Nam Kim, Seung Woo Suh, Kevin G. Becker, Yoon S. Cho-Chung, Meyoung Kon Kim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Osteoporosis associated with estrogen deficiency is defined as an abnormal decrease in bone mass leading to an increased fracture risk. Genistein (GEN), as a phytoestrogen, is a type of soybean-derived isoflavone that possesses structural similarity to estrogen. In this study, we assessed the effect of GEN in ovariectomized (OVX) mice. To determine the effect of GEN on bone metabolism, we investigated gene expression profiles using a radioactive cDNA microarray. Eight-week-old female mice were either sham operated (SHAM) or OVX. From 1 week after the operation, OVX mice were injected daily with intraperitoneal GEN (0.1, 0.5, 1.5 and 3.0 mg/day) or 17β-estradiol (E₂, 0.03 μg/day) for 4 weeks. A cDNA microarray was used to evaluate changes in the expression of 1,152 genes. OVX mice showed bone mineral density (BMD) loss versus SHAM mice (5.8±0.4 vs. 6.9±0.6 mg/cm²). However, femur BMDs were completely restored by GEN and by E₂ administration in OVX mice. Serum osteocalcin in OVX mice treated with 0.5 mg/day of GEN was 1.6-fold (44.30±5.73 ng/ml) higher than that in untreated mice. GEN treatment up-regulated 38 genes (e.g., mitogen-activated protein kinase 10) and down-regulated 18 (e.g., matrix metalloproteinase 13). Moreover, GEN was found to have a protective effect on bone loss caused by estrogen deficiency in OVX mice. The present study suggests that GEN modulates bone metabolism-related gene expression, including calciotropic receptor, cytokines, growth factors and bone matrix proteins.

Original language	English
Pages (from-to)	157-164
Number of pages	8
Journal	Journal of Nutritional Biochemistry
Volume	17
Issue number	3
DOIs	https://doi.org/10.1016/j.jnutbio.2005.06.002
Publication status	Published - 2006 Mar

Bibliographical note

Funding Information:
The authors thank Dr. Yoon S. Cho-Chung (Cellular Biochemistry Section, Basic Research Laboratory, CCR, NCI, NIH, Bethesda, MD) and Dr. Kevin G. Becker (DNA Array Unit, NIA, NIH, Baltimore, MD) for valuable advices on cDNA microarray. Also, we thank Yeon mi Ryu and Hye won Kim (Department of Biochemistry & Molecular Biology, Korea University Medical School, Seoul, Korea) for valuable advice on this paper. This study was supported by a grant from the Medical Research Center for Environmental Toxicogenomic and Proteomics, funded by the Korea Science and Engineering Foundations and Ministry of Science & Technology, a grant from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (Hmp-00-GN-01-0002 and KPGRN-R-04), a Korea Institute of Science & Technology Evaluation and Planning (KISTEP) and Ministry of Science & Technology (MOST), the Korean government, through its National Nuclear Technology Program, and grant no. R01-2001-000-00212-0 from the Basic Research Program of the Korea Science & Engineering Foundation.

Keywords

Bone metabolism
Gene profiling
Genistein
Osteoporosis
cDNA microarray

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology
Nutrition and Dietetics
Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.jnutbio.2005.06.002

Cite this

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title = "Effect of genistein on the expression of bone metabolism genes in ovariectomized mice using a cDNA microarray",

abstract = "Osteoporosis associated with estrogen deficiency is defined as an abnormal decrease in bone mass leading to an increased fracture risk. Genistein (GEN), as a phytoestrogen, is a type of soybean-derived isoflavone that possesses structural similarity to estrogen. In this study, we assessed the effect of GEN in ovariectomized (OVX) mice. To determine the effect of GEN on bone metabolism, we investigated gene expression profiles using a radioactive cDNA microarray. Eight-week-old female mice were either sham operated (SHAM) or OVX. From 1 week after the operation, OVX mice were injected daily with intraperitoneal GEN (0.1, 0.5, 1.5 and 3.0 mg/day) or 17β-estradiol (E2, 0.03 μg/day) for 4 weeks. A cDNA microarray was used to evaluate changes in the expression of 1,152 genes. OVX mice showed bone mineral density (BMD) loss versus SHAM mice (5.8±0.4 vs. 6.9±0.6 mg/cm2). However, femur BMDs were completely restored by GEN and by E2 administration in OVX mice. Serum osteocalcin in OVX mice treated with 0.5 mg/day of GEN was 1.6-fold (44.30±5.73 ng/ml) higher than that in untreated mice. GEN treatment up-regulated 38 genes (e.g., mitogen-activated protein kinase 10) and down-regulated 18 (e.g., matrix metalloproteinase 13). Moreover, GEN was found to have a protective effect on bone loss caused by estrogen deficiency in OVX mice. The present study suggests that GEN modulates bone metabolism-related gene expression, including calciotropic receptor, cytokines, growth factors and bone matrix proteins.",

keywords = "Bone metabolism, Gene profiling, Genistein, Osteoporosis, cDNA microarray",

author = "Pie, {Jae Eun} and Park, {Jin Hee} and Park, {Yoon Hee} and Ryu, {Yeon Mi} and Kim, {Ki Nam} and Suh, {Seung Woo} and Becker, {Kevin G.} and Cho-Chung, {Yoon S.} and Kim, {Meyoung Kon}",

note = "Funding Information: The authors thank Dr. Yoon S. Cho-Chung (Cellular Biochemistry Section, Basic Research Laboratory, CCR, NCI, NIH, Bethesda, MD) and Dr. Kevin G. Becker (DNA Array Unit, NIA, NIH, Baltimore, MD) for valuable advices on cDNA microarray. Also, we thank Yeon mi Ryu and Hye won Kim (Department of Biochemistry & Molecular Biology, Korea University Medical School, Seoul, Korea) for valuable advice on this paper. This study was supported by a grant from the Medical Research Center for Environmental Toxicogenomic and Proteomics, funded by the Korea Science and Engineering Foundations and Ministry of Science & Technology, a grant from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (Hmp-00-GN-01-0002 and KPGRN-R-04), a Korea Institute of Science & Technology Evaluation and Planning (KISTEP) and Ministry of Science & Technology (MOST), the Korean government, through its National Nuclear Technology Program, and grant no. R01-2001-000-00212-0 from the Basic Research Program of the Korea Science & Engineering Foundation.",

year = "2006",

month = mar,

doi = "10.1016/j.jnutbio.2005.06.002",

language = "English",

volume = "17",

pages = "157--164",

journal = "Journal of Nutritional Biochemistry",

issn = "0955-2863",

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TY - JOUR

T1 - Effect of genistein on the expression of bone metabolism genes in ovariectomized mice using a cDNA microarray

AU - Pie, Jae Eun

AU - Park, Jin Hee

AU - Park, Yoon Hee

AU - Ryu, Yeon Mi

AU - Kim, Ki Nam

AU - Suh, Seung Woo

AU - Becker, Kevin G.

AU - Cho-Chung, Yoon S.

AU - Kim, Meyoung Kon

N1 - Funding Information: The authors thank Dr. Yoon S. Cho-Chung (Cellular Biochemistry Section, Basic Research Laboratory, CCR, NCI, NIH, Bethesda, MD) and Dr. Kevin G. Becker (DNA Array Unit, NIA, NIH, Baltimore, MD) for valuable advices on cDNA microarray. Also, we thank Yeon mi Ryu and Hye won Kim (Department of Biochemistry & Molecular Biology, Korea University Medical School, Seoul, Korea) for valuable advice on this paper. This study was supported by a grant from the Medical Research Center for Environmental Toxicogenomic and Proteomics, funded by the Korea Science and Engineering Foundations and Ministry of Science & Technology, a grant from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (Hmp-00-GN-01-0002 and KPGRN-R-04), a Korea Institute of Science & Technology Evaluation and Planning (KISTEP) and Ministry of Science & Technology (MOST), the Korean government, through its National Nuclear Technology Program, and grant no. R01-2001-000-00212-0 from the Basic Research Program of the Korea Science & Engineering Foundation.

PY - 2006/3

Y1 - 2006/3

N2 - Osteoporosis associated with estrogen deficiency is defined as an abnormal decrease in bone mass leading to an increased fracture risk. Genistein (GEN), as a phytoestrogen, is a type of soybean-derived isoflavone that possesses structural similarity to estrogen. In this study, we assessed the effect of GEN in ovariectomized (OVX) mice. To determine the effect of GEN on bone metabolism, we investigated gene expression profiles using a radioactive cDNA microarray. Eight-week-old female mice were either sham operated (SHAM) or OVX. From 1 week after the operation, OVX mice were injected daily with intraperitoneal GEN (0.1, 0.5, 1.5 and 3.0 mg/day) or 17β-estradiol (E2, 0.03 μg/day) for 4 weeks. A cDNA microarray was used to evaluate changes in the expression of 1,152 genes. OVX mice showed bone mineral density (BMD) loss versus SHAM mice (5.8±0.4 vs. 6.9±0.6 mg/cm2). However, femur BMDs were completely restored by GEN and by E2 administration in OVX mice. Serum osteocalcin in OVX mice treated with 0.5 mg/day of GEN was 1.6-fold (44.30±5.73 ng/ml) higher than that in untreated mice. GEN treatment up-regulated 38 genes (e.g., mitogen-activated protein kinase 10) and down-regulated 18 (e.g., matrix metalloproteinase 13). Moreover, GEN was found to have a protective effect on bone loss caused by estrogen deficiency in OVX mice. The present study suggests that GEN modulates bone metabolism-related gene expression, including calciotropic receptor, cytokines, growth factors and bone matrix proteins.

AB - Osteoporosis associated with estrogen deficiency is defined as an abnormal decrease in bone mass leading to an increased fracture risk. Genistein (GEN), as a phytoestrogen, is a type of soybean-derived isoflavone that possesses structural similarity to estrogen. In this study, we assessed the effect of GEN in ovariectomized (OVX) mice. To determine the effect of GEN on bone metabolism, we investigated gene expression profiles using a radioactive cDNA microarray. Eight-week-old female mice were either sham operated (SHAM) or OVX. From 1 week after the operation, OVX mice were injected daily with intraperitoneal GEN (0.1, 0.5, 1.5 and 3.0 mg/day) or 17β-estradiol (E2, 0.03 μg/day) for 4 weeks. A cDNA microarray was used to evaluate changes in the expression of 1,152 genes. OVX mice showed bone mineral density (BMD) loss versus SHAM mice (5.8±0.4 vs. 6.9±0.6 mg/cm2). However, femur BMDs were completely restored by GEN and by E2 administration in OVX mice. Serum osteocalcin in OVX mice treated with 0.5 mg/day of GEN was 1.6-fold (44.30±5.73 ng/ml) higher than that in untreated mice. GEN treatment up-regulated 38 genes (e.g., mitogen-activated protein kinase 10) and down-regulated 18 (e.g., matrix metalloproteinase 13). Moreover, GEN was found to have a protective effect on bone loss caused by estrogen deficiency in OVX mice. The present study suggests that GEN modulates bone metabolism-related gene expression, including calciotropic receptor, cytokines, growth factors and bone matrix proteins.

KW - Bone metabolism

KW - Gene profiling

KW - Genistein

KW - Osteoporosis

KW - cDNA microarray

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M3 - Article

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SN - 0955-2863

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EP - 164

JO - Journal of Nutritional Biochemistry

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IS - 3

ER -

Effect of genistein on the expression of bone metabolism genes in ovariectomized mice using a cDNA microarray

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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