Effect of microencapsulated precipitants of lactobacillus casei ATCC 393 on helicobacter pylori eradication

J. A. Ko; H. J. Lim; H. J. Park

doi:10.1016/j.procbio.2010.10.012

Effect of microencapsulated precipitants of lactobacillus casei ATCC 393 on helicobacter pylori eradication

J. A. Ko, H. J. Lim, H. J. Park

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Precipitants of Lactobacillus casei ATCC 393-loaded chitosan microspheres were prepared by electrostatic interaction between chitosan (CS) and poly-γ-glutamic acid (γ-PGA). The loading efficiency was approximately 80%, and the zeta potential of the microspheres became more positively charged as the molar ratio of the chitosan amino group was increased. The particles were confirmed to have a spherical shape by optical microscopy. A release test using simulated gastric fluid (SGF) was also performed. The results show that the precipitants were gradually released from the CS-γ-PGA microspheres at a rate of almost 80% after 60 min. Both the inhibition of Helicobacter pylori growth and the anti-adhesive activity of encapsulated precipitants were maintained after exposure to SGF. These results indicate that CS-γ-PGA microspheres may provide a delivery system for the effective eradication of H. pylori.

Original language	English
Pages (from-to)	631-635
Number of pages	5
Journal	Process Biochemistry
Volume	46
Issue number	3
DOIs	https://doi.org/10.1016/j.procbio.2010.10.012
Publication status	Published - 2011 Mar

Bibliographical note

Funding Information:
This study was supported by a Grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea ( A050376 ).

Keywords

Chitosan
Helicobacter pylori
Lactobacillus casei ATCC 393
Microspheres
Poly-γ-glutamic

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

Access to Document

10.1016/j.procbio.2010.10.012

Cite this

@article{3564b8f77ba04c9c856f04969922f658,

title = "Effect of microencapsulated precipitants of lactobacillus casei ATCC 393 on helicobacter pylori eradication",

abstract = "Precipitants of Lactobacillus casei ATCC 393-loaded chitosan microspheres were prepared by electrostatic interaction between chitosan (CS) and poly-γ-glutamic acid (γ-PGA). The loading efficiency was approximately 80%, and the zeta potential of the microspheres became more positively charged as the molar ratio of the chitosan amino group was increased. The particles were confirmed to have a spherical shape by optical microscopy. A release test using simulated gastric fluid (SGF) was also performed. The results show that the precipitants were gradually released from the CS-γ-PGA microspheres at a rate of almost 80% after 60 min. Both the inhibition of Helicobacter pylori growth and the anti-adhesive activity of encapsulated precipitants were maintained after exposure to SGF. These results indicate that CS-γ-PGA microspheres may provide a delivery system for the effective eradication of H. pylori.",

keywords = "Chitosan, Helicobacter pylori, Lactobacillus casei ATCC 393, Microspheres, Poly-γ-glutamic",

author = "Ko, {J. A.} and Lim, {H. J.} and Park, {H. J.}",

note = "Funding Information: This study was supported by a Grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea ( A050376 ).",

year = "2011",

month = mar,

doi = "10.1016/j.procbio.2010.10.012",

language = "English",

volume = "46",

pages = "631--635",

journal = "Process Biochemistry",

issn = "1359-5113",

publisher = "Elsevier Ltd",

number = "3",

}

TY - JOUR

T1 - Effect of microencapsulated precipitants of lactobacillus casei ATCC 393 on helicobacter pylori eradication

AU - Ko, J. A.

AU - Lim, H. J.

AU - Park, H. J.

N1 - Funding Information: This study was supported by a Grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea ( A050376 ).

PY - 2011/3

Y1 - 2011/3

N2 - Precipitants of Lactobacillus casei ATCC 393-loaded chitosan microspheres were prepared by electrostatic interaction between chitosan (CS) and poly-γ-glutamic acid (γ-PGA). The loading efficiency was approximately 80%, and the zeta potential of the microspheres became more positively charged as the molar ratio of the chitosan amino group was increased. The particles were confirmed to have a spherical shape by optical microscopy. A release test using simulated gastric fluid (SGF) was also performed. The results show that the precipitants were gradually released from the CS-γ-PGA microspheres at a rate of almost 80% after 60 min. Both the inhibition of Helicobacter pylori growth and the anti-adhesive activity of encapsulated precipitants were maintained after exposure to SGF. These results indicate that CS-γ-PGA microspheres may provide a delivery system for the effective eradication of H. pylori.

AB - Precipitants of Lactobacillus casei ATCC 393-loaded chitosan microspheres were prepared by electrostatic interaction between chitosan (CS) and poly-γ-glutamic acid (γ-PGA). The loading efficiency was approximately 80%, and the zeta potential of the microspheres became more positively charged as the molar ratio of the chitosan amino group was increased. The particles were confirmed to have a spherical shape by optical microscopy. A release test using simulated gastric fluid (SGF) was also performed. The results show that the precipitants were gradually released from the CS-γ-PGA microspheres at a rate of almost 80% after 60 min. Both the inhibition of Helicobacter pylori growth and the anti-adhesive activity of encapsulated precipitants were maintained after exposure to SGF. These results indicate that CS-γ-PGA microspheres may provide a delivery system for the effective eradication of H. pylori.

KW - Chitosan

KW - Helicobacter pylori

KW - Lactobacillus casei ATCC 393

KW - Microspheres

KW - Poly-γ-glutamic

UR - http://www.scopus.com/inward/record.url?scp=79951508236&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2010.10.012

DO - 10.1016/j.procbio.2010.10.012

M3 - Article

AN - SCOPUS:79951508236

SN - 1359-5113

VL - 46

SP - 631

EP - 635

JO - Process Biochemistry

JF - Process Biochemistry

IS - 3

ER -

Effect of microencapsulated precipitants of lactobacillus casei ATCC 393 on helicobacter pylori eradication

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this