Abstract
Precipitants of Lactobacillus casei ATCC 393-loaded chitosan microspheres were prepared by electrostatic interaction between chitosan (CS) and poly-γ-glutamic acid (γ-PGA). The loading efficiency was approximately 80%, and the zeta potential of the microspheres became more positively charged as the molar ratio of the chitosan amino group was increased. The particles were confirmed to have a spherical shape by optical microscopy. A release test using simulated gastric fluid (SGF) was also performed. The results show that the precipitants were gradually released from the CS-γ-PGA microspheres at a rate of almost 80% after 60 min. Both the inhibition of Helicobacter pylori growth and the anti-adhesive activity of encapsulated precipitants were maintained after exposure to SGF. These results indicate that CS-γ-PGA microspheres may provide a delivery system for the effective eradication of H. pylori.
Original language | English |
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Pages (from-to) | 631-635 |
Number of pages | 5 |
Journal | Process Biochemistry |
Volume | 46 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2011 Mar |
Bibliographical note
Funding Information:This study was supported by a Grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea ( A050376 ).
Keywords
- Chitosan
- Helicobacter pylori
- Lactobacillus casei ATCC 393
- Microspheres
- Poly-γ-glutamic
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology