Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages

Suk Jun Lee; Ji Hye Lee; Han Hyung Lee; Seul Lee; Sae Hun Kim; Taehoon Chun; Jee Young Imm

doi:10.1007/s10068-011-0072-z

Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages

Suk Jun Lee, Ji Hye Lee, Han Hyung Lee, Seul Lee, Sae Hun Kim, Taehoon Chun, Jee Young Imm

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

The anti-inflammatory effect of mung bean ethanol extract on lipopolysaccharide (LPS) stimulated macrophages (J774) was evaluated. The mung bean extract was separated into 5 fractions by normal phase silica gel column chromatography and the mRNA expressions of pro-inflammatory cytokines were examined after incubation with each fraction in LPS stimulated macrophages. All pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-12β, tumor necrosis factor (TNF)-α, and inducible NO synthase (iNOS) were remarkably decreased in the cells treated with 3.7 mg/mL of F3 fraction. The active F3 fraction did not show any cytotoxcity according to propidium iodide staining and no inhibitory effects on J774 cell proliferation were observed by MTT assay. The active fraction contained gallic acid, vitexin, and isovitexin as major components.

Original language	English
Pages (from-to)	519-524
Number of pages	6
Journal	Food Science and Biotechnology
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/s10068-011-0072-z
Publication status	Published - 2011 Apr

Bibliographical note

Funding Information:
Acknowledgments This research was supported by Technology Development Program for Food (109141-03), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

Keywords

Inflammation
Macrophage
Mung bean ethanol extract
Pro-inflammatory cytokine

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

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10.1007/s10068-011-0072-z

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title = "Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages",

abstract = "The anti-inflammatory effect of mung bean ethanol extract on lipopolysaccharide (LPS) stimulated macrophages (J774) was evaluated. The mung bean extract was separated into 5 fractions by normal phase silica gel column chromatography and the mRNA expressions of pro-inflammatory cytokines were examined after incubation with each fraction in LPS stimulated macrophages. All pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-12β, tumor necrosis factor (TNF)-α, and inducible NO synthase (iNOS) were remarkably decreased in the cells treated with 3.7 mg/mL of F3 fraction. The active F3 fraction did not show any cytotoxcity according to propidium iodide staining and no inhibitory effects on J774 cell proliferation were observed by MTT assay. The active fraction contained gallic acid, vitexin, and isovitexin as major components.",

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note = "Funding Information: Acknowledgments This research was supported by Technology Development Program for Food (109141-03), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.",

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AU - Lee, Ji Hye

AU - Lee, Han Hyung

AU - Lee, Seul

AU - Kim, Sae Hun

AU - Chun, Taehoon

AU - Imm, Jee Young

N1 - Funding Information: Acknowledgments This research was supported by Technology Development Program for Food (109141-03), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

PY - 2011/4

Y1 - 2011/4

N2 - The anti-inflammatory effect of mung bean ethanol extract on lipopolysaccharide (LPS) stimulated macrophages (J774) was evaluated. The mung bean extract was separated into 5 fractions by normal phase silica gel column chromatography and the mRNA expressions of pro-inflammatory cytokines were examined after incubation with each fraction in LPS stimulated macrophages. All pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-12β, tumor necrosis factor (TNF)-α, and inducible NO synthase (iNOS) were remarkably decreased in the cells treated with 3.7 mg/mL of F3 fraction. The active F3 fraction did not show any cytotoxcity according to propidium iodide staining and no inhibitory effects on J774 cell proliferation were observed by MTT assay. The active fraction contained gallic acid, vitexin, and isovitexin as major components.

AB - The anti-inflammatory effect of mung bean ethanol extract on lipopolysaccharide (LPS) stimulated macrophages (J774) was evaluated. The mung bean extract was separated into 5 fractions by normal phase silica gel column chromatography and the mRNA expressions of pro-inflammatory cytokines were examined after incubation with each fraction in LPS stimulated macrophages. All pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-12β, tumor necrosis factor (TNF)-α, and inducible NO synthase (iNOS) were remarkably decreased in the cells treated with 3.7 mg/mL of F3 fraction. The active F3 fraction did not show any cytotoxcity according to propidium iodide staining and no inhibitory effects on J774 cell proliferation were observed by MTT assay. The active fraction contained gallic acid, vitexin, and isovitexin as major components.

KW - Inflammation

KW - Macrophage

KW - Mung bean ethanol extract

KW - Pro-inflammatory cytokine

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Effect of mung bean ethanol extract on pro-inflammtory cytokines in LPS stimulated macrophages

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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