Abstract
Background P2Y1 and P2Y12 receptors are expressed in platelet membranes and are involved in ADP-induced platelet aggregation. Genetic polymorphisms of P2Y1 and P2Y12 play a major role in the variation of ADP-induced platelet aggregation and in response in antiplatelet therapy. Objective To evaluate the allele frequencies of P2Y1 and P2Y12 genetic polymorphisms in a Korean population and to assess their role in ADP (5 μmol/L)-induced maximal platelet aggregation. Methods P2Y1 (c.1622A > G) and P2Y12 (i-139C > T, i-744 T > C, i-ins801, c.52G > T, c.34C > T) polymorphisms were analyzed in 158 Korean healthy participants using pyrosequencing methods. Their ADP-induced maximal platelet aggregation was assessed by the turbidometric method. Results The observed allele frequencies of P2Y1 and P2Y12 were as follows: 0.3101 for P2Y1 c.1622A > G; 0.1804 for P2Y12 i-139C > T, 0.1804 for i-744 T > C, 0.1804 for i-801insA, 0.1266 for P2Y12 c.52G > T, and 0.2658 for P2Y12 c.34C > T. ADP-induced maximal platelet aggregation was not influenced by the P2Y 1 c.1622A > G polymorphism and was also not affected by three intronic P2Y12 polymorphisms and the P2Y12 c.34C > T polymorphism. However, the P2Y12 c.52G > T polymorphism caused a substantial difference in ADP-induced maximal platelet aggregation (62.75% for c.52GG, 66.27% for c.52GT, and 80.60% for c.52TT; P = 0.0092). Conclusions The P2Y1 and P2Y12 genes were very polymorphic in a Korean population. Three intronic P2Y12 polymorphisms (i-139C > T, i-744 T > C, i-801insA) were in complete linkage disequilibrium but not with the c.52C > T polymorphism in this population. Maximal platelet aggregation in response to ADP is associated with the c.52C > T polymorphism but not with the three intronic polymorphisms or the P2Y1 c.1622A > T polymorphism.
Original language | English |
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Pages (from-to) | 221-226 |
Number of pages | 6 |
Journal | Thrombosis Research |
Volume | 132 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2013 Aug |
Externally published | Yes |
Bibliographical note
Funding Information:This study was supported by a grant from the Korea Health Technology R&D Project, Ministry for Health, Welfare and Family Affairs , Republic of Korea ( A070001 ).
Keywords
- ADP
- P2Y
- P2Y
- Platelet aggregation
- Polymorphism
ASJC Scopus subject areas
- Hematology