Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Hyun Do Kim; Eun Hee Bae; Ick Chan Kwon; Ravindra Ramsurat Pal; Jae Do Nam; Doo Sung Lee

doi:10.1016/j.biomaterials.2003.09.011

Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Hyun Do Kim, Eun Hee Bae, Ick Chan Kwon, Ravindra Ramsurat Pal, Jae Do Nam, Doo Sung Lee

Research output: Contribution to journal › Article › peer-review

146 Citations (Scopus)

Abstract

A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

Original language	English
Pages (from-to)	2319-2329
Number of pages	11
Journal	Biomaterials
Volume	25
Issue number	12
DOIs	https://doi.org/10.1016/j.biomaterials.2003.09.011
Publication status	Published - 2004 May

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Research Foundation (no. KRF-2001-005-E00006).

Keywords

MC3T3-E1 cell
Macroporous scaffold
PEG-PLLA diblock copolymer
PLLA
Thermally induced phase separation (TIPS)

ASJC Scopus subject areas

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

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10.1016/j.biomaterials.2003.09.011

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title = "Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation",

abstract = "A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.",

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author = "Kim, {Hyun Do} and Bae, {Eun Hee} and Kwon, {Ick Chan} and Pal, {Ravindra Ramsurat} and Nam, {Jae Do} and Lee, {Doo Sung}",

note = "Funding Information: This work was supported by a grant from the Korea Research Foundation (no. KRF-2001-005-E00006).",

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doi = "10.1016/j.biomaterials.2003.09.011",

language = "English",

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AU - Bae, Eun Hee

AU - Kwon, Ick Chan

AU - Pal, Ravindra Ramsurat

AU - Nam, Jae Do

AU - Lee, Doo Sung

N1 - Funding Information: This work was supported by a grant from the Korea Research Foundation (no. KRF-2001-005-E00006).

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N2 - A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

AB - A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

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Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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