Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Hyun Do Kim; Eun Hee Bae; Ick Chan Kwon; Ravindra Ramsurat Pal; Jae Do Nam; Doo Sung Lee

doi:10.1016/j.biomaterials.2003.09.011

Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Hyun Do Kim, Eun Hee Bae, Ick Chan Kwon, Ravindra Ramsurat Pal, Jae Do Nam, Doo Sung Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

146 Citations (Scopus)

Abstract

A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

Original language	English
Pages (from-to)	2319-2329
Number of pages	11
Journal	Biomaterials
Volume	25
Issue number	12
DOIs	https://doi.org/10.1016/j.biomaterials.2003.09.011
Publication status	Published - 2004 May

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Research Foundation (no. KRF-2001-005-E00006).

Keywords

MC3T3-E1 cell
Macroporous scaffold
PEG-PLLA diblock copolymer
PLLA
Thermally induced phase separation (TIPS)

ASJC Scopus subject areas

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

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10.1016/j.biomaterials.2003.09.011

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title = "Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation",

abstract = "A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt\% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.",

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author = "Kim, \{Hyun Do\} and Bae, \{Eun Hee\} and Kwon, \{Ick Chan\} and Pal, \{Ravindra Ramsurat\} and Nam, \{Jae Do\} and Lee, \{Doo Sung\}",

note = "Funding Information: This work was supported by a grant from the Korea Research Foundation (no. KRF-2001-005-E00006).",

year = "2004",

month = may,

doi = "10.1016/j.biomaterials.2003.09.011",

language = "English",

volume = "25",

pages = "2319--2329",

journal = "Biomaterials",

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AU - Kim, Hyun Do

AU - Bae, Eun Hee

AU - Kwon, Ick Chan

AU - Pal, Ravindra Ramsurat

AU - Nam, Jae Do

AU - Lee, Doo Sung

N1 - Funding Information: This work was supported by a grant from the Korea Research Foundation (no. KRF-2001-005-E00006).

PY - 2004/5

Y1 - 2004/5

N2 - A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

AB - A regular and highly interconnected macroporous poly(L-lactic acid) (PLLA) scaffold was fabricated from a PLLA-dioxane-water ternary system with added polyethylene glycol (PEG)-PLLA diblock using thermally induced phase separation (TIPS). The morphology of the scaffold was investigated in detail by controlling the following TIPS parameters: quenching temperature, aging time, polymer concentration, molecular structure, and diblock concentration. The phase diagram was assessed visually on the basis of the turbidity. The cloud-point curve shifted to higher temperatures with increasing PEG content in the additives (PEG-PLLA diblocks), due to a stronger interaction between PEG and water in solution. The addition of diblock series (0.5wt% in solution) stabilized interconnections of pores at a later stage without segregation or sedimentation. The pore size of the scaffold could be easily controlled in the range 50-300μm. A macroporous PLLA scaffold was used to study an MC3T3-E1 cell (an osteoblast-like cell) culture. The cells successfully proliferated in the PLLA scaffold in the presence of added PEG-PLLA diblock for 4 weeks.

KW - MC3T3-E1 cell

KW - Macroporous scaffold

KW - PEG-PLLA diblock copolymer

KW - PLLA

KW - Thermally induced phase separation (TIPS)

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U2 - 10.1016/j.biomaterials.2003.09.011

DO - 10.1016/j.biomaterials.2003.09.011

M3 - Article

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AN - SCOPUS:1642515777

SN - 0142-9612

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JO - Biomaterials

JF - Biomaterials

IS - 12

ER -

Effect of PEG-PLLA diblock copolymer on macroporous PLLA scaffolds by thermally induced phase separation

Abstract

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Keywords

ASJC Scopus subject areas

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