Effect of porcine placenta extract from subcritical water extraction on photodamage in human keratinocytes

Yooheon Park, Bok Kyung Han, Hyeon Son Choi, Yang Hee Hong, Eun Young Jung, Hyung Joo Suh

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The objective of this study was to evaluated the photoprotective effects of porcine placenta extract (PPE) on ultraviolet B (UVB)-induced oxidative stress in human keratinocytes (HaCaT) to evaluate its functional activities as a skin food ingredient. PPE prepared by subcritical water extraction was termed SPE, and subsequently digested by enzymes to prepare E-SPE. Increased intracellular reactive oxygen species (ROS) levels (192.0%) induced by UVB were decreased by SPE and E-SPE. SPE had more effective ROS scavenging activity than E-SPE treatment. UVB treatment increased expression of tissue inhibitor of metalloproteinase 1 (TIMP-1), and this elevated expression was decreased by E-SPE treatment. High-dose treatment with E-SPE (50 and 100 μg/mL) reduced TIMP-1 expression levels of UVB-C (control) to 33.5 and 34.6%, respectively. In contrast, at low SPE doses (1 and 10 μg/mL), the treatment slightly decreased TIMP-1 expression levels to 73.3% and 71.3% of UVB-C, respectively. In conclusion, the present study demonstrated the protective effect of SPE and E-SPE against UVB damage in keratinocytes via ROS scavenging, down-regulating MMP-2 expression and up-regulating TIMP-1 expression. This highlights the potential for SPE as an ingredient in the preparation of functional food against photoaging.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalKorean Journal for Food Science of Animal Resources
Issue number2
Publication statusPublished - 2015 Apr 1


  • MMP-2
  • Porcine placenta extract
  • ROS
  • Subcritical water extraction
  • TIMP-1
  • UVB

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology


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