Effect of rhamnogalacturonan-I-rich polysaccharides isolated from crabapple hydrolysates on IL-1β-induced inflammation in intestinal epithelial cells

Hyun Young Shin, Yeon Suk Kim, Kwang Soon Shin, Sung Hee Han, Hyung Joo Suh, Kwang Won Yu

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to investigate the structural characteristics and intracellular mechanisms of polysaccharides (MP-PE-I) purified from a crabapple (Malus prunifolia) enzymatic hydrolysate (MP-PE). Activity-guided fractionation revealed that MP-PE-I was the active moiety and significantly reduced the production and gene expression of pro-inflammatory factors in interleukin (IL)-1β-treated intestinal epithelial cells (Caco-2). Moreover, MP-PE-I downregulated the phosphorylation and nuclear localization of proteins involved in the mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) pathways, as evidenced by immunoblotting and immunofluorescence analysis. In antagonistic studies with specific inhibitors of the MAPK and NF-κB pathways, IL-6 inhibition was significantly regulated by p38; IL-8 by IκBα, JNK, and p38; and monocyte chemoattractant protein-1 (MCP-1) by JNK, p38, and ERK. Additionally, MP-PE-I significantly decreased the mRNA and protein expression of IL-1 receptor type 1. Chemical and structural characteristic analyses showed that MP-PE-I is a polysaccharide rich in rhamnogalacturonan (RG)-I and plays a crucial role in intestinal immunomodulation. To our knowledge, this is the first study to demonstrate the intestinal immunomodulatory activity, intracellular mechanisms, and structural characteristics of RG-I-rich polysaccharides isolated from crabapples.

Original languageEnglish
Article number134240
JournalInternational Journal of Biological Macromolecules
Volume277
DOIs
Publication statusPublished - 2024 Oct

Bibliographical note

Publisher Copyright:
© 2024 Elsevier B.V.

Keywords

  • Crabapple
  • Enzymatic hydrolysate
  • Intestinal immunomodulatory activity
  • Malus prunifolia
  • Rhamnogalacturonan-I polysaccharide

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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