Effect of rhBMP-2 immobilized anorganic bovine bone matrix on bone regeneration

Jung Bo Huh, June Jip Yang, Kyung Hee Choi, Ji Hyeon Bae, Jeong Yeol Lee, Sung Eun Kim, Sang Wan Shin

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Anorganic bovine bone matrix (Bio-Oss®) has been used for a long time for bone graft regeneration, but has poor osteoinductive capability. The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) has been suggested to overcome this limitation of Bio-Oss®. In the present study, heparin-mediated rhBMP-2 was combined with Bio-Oss® in animal experiments to investigate bone formation performance; heparin was used to control rhBMP-2 release. Two calvarial defects (8 mm diameter) were formed in a white rabbit model and then implanted or not (controls) with Bio-Oss® or BMP-2/Bio-Oss®. The Bio-Oss® and BMP-2/Bio-Oss® groups had significantly greater new bone areas (expressed as percentages of augmented areas) than the non-implanted controls at four and eight weeks after surgery, and the BMP-2/Bio-Oss® group (16.50 ± 2.87 (n = 6)) had significantly greater new bone areas than the Bio-Oss® group (9.43 ± 3.73 (n = 6)) at four weeks. These findings suggest that rhBMP-2 treated heparinized Bio-Oss® markedly enhances bone regeneration.

Original languageEnglish
Pages (from-to)16034-16052
Number of pages19
JournalInternational journal of molecular sciences
Issue number7
Publication statusPublished - 2015 Jul 14

Bibliographical note

Publisher Copyright:
© 2015 by the authors; licensee MDPI, Basel, Switzerland.


  • Anorganic bovine bone
  • Bio-Oss®
  • Bone regeneration
  • RhBMP-2

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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