TY - JOUR
T1 - Effect of specific production rate of recombinant protein on multimerization of plasmid vector and gene expression level
AU - Saraswat, Vibhor
AU - Kim, Dae Young
AU - Lee, Jeewon
AU - Park, Young Hoon
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/10/15
Y1 - 1999/10/15
N2 - In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin-2, G3.IL-2. A maximum concentration of G3.IL-2 (11.25 g l-1) was achieved in the induced recombinant culture growing at the rate of 0.056 h-1. A steep decrease in the expression level of G3.IL-2 was observed at the post-induction specific growth rates higher than its optimal value (0.056 h-1). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post-induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture. Copyright (C) 1999 Federation of European Microbiological Societies.
AB - In fed-batch cultures of recombinant Escherichia coli BL21(DE3)[pT7-G3IL2] at high cell concentration, the post-induction specific growth rate was carefully regulated by controlled medium feed to maximize the synthesis level of recombinant fusion interleukin-2, G3.IL-2. A maximum concentration of G3.IL-2 (11.25 g l-1) was achieved in the induced recombinant culture growing at the rate of 0.056 h-1. A steep decrease in the expression level of G3.IL-2 was observed at the post-induction specific growth rates higher than its optimal value (0.056 h-1). In the induced recombinant cultures, plasmid multimerization was observed and highly dependent on specific growth and production rate: a higher post-induction specific growth rate and an increased specific production rate tended to significantly promote it much further. Moreover, plasmid stability was found to decrease rapidly in a faster growing culture. Copyright (C) 1999 Federation of European Microbiological Societies.
KW - Escherichia coli
KW - Plasmid multimerization
KW - Recombinant human interleukin-2
KW - Specific production rate
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U2 - 10.1016/S0378-1097(99)00436-X
DO - 10.1016/S0378-1097(99)00436-X
M3 - Article
C2 - 10518739
AN - SCOPUS:0032824865
SN - 0378-1097
VL - 179
SP - 367
EP - 373
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -