Effect of tobacco compounds on gene expression profiles in human epithelial cells

Sung Hwa Sohn; Jaebum Lee; Ki Nam Kim; In kyoung Kim; Meyoung Kon Kim

doi:10.1016/j.etap.2008.09.005

Effect of tobacco compounds on gene expression profiles in human epithelial cells

Sung Hwa Sohn, Jaebum Lee, Ki Nam Kim, In kyoung Kim, Meyoung Kon Kim

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.

Original language	English
Pages (from-to)	111-119
Number of pages	9
Journal	Environmental Toxicology and Pharmacology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.etap.2008.09.005
Publication status	Published - 2009 Jan
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R13-2003-016-03002-0) and Korea Ministry of Environment as “The Eco-technopia 21 project (No. 2008-09002-0012-0)”.

Keywords

2-Naphthylamine
B(a)P
Gene expression
Microarray
Nicotine

ASJC Scopus subject areas

Toxicology
Pharmacology
Health, Toxicology and Mutagenesis

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10.1016/j.etap.2008.09.005

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title = "Effect of tobacco compounds on gene expression profiles in human epithelial cells",

abstract = "This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.",

keywords = "2-Naphthylamine, B(a)P, Gene expression, Microarray, Nicotine",

author = "Sohn, {Sung Hwa} and Jaebum Lee and Kim, {Ki Nam} and Kim, {In kyoung} and Kim, {Meyoung Kon}",

note = "Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R13-2003-016-03002-0) and Korea Ministry of Environment as “The Eco-technopia 21 project (No. 2008-09002-0012-0)”. ",

year = "2009",

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doi = "10.1016/j.etap.2008.09.005",

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AU - Sohn, Sung Hwa

AU - Lee, Jaebum

AU - Kim, Ki Nam

AU - Kim, In kyoung

AU - Kim, Meyoung Kon

N1 - Funding Information: This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R13-2003-016-03002-0) and Korea Ministry of Environment as “The Eco-technopia 21 project (No. 2008-09002-0012-0)”.

PY - 2009/1

Y1 - 2009/1

N2 - This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.

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Effect of tobacco compounds on gene expression profiles in human epithelial cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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