Effect of tobacco compounds on gene expression profiles in human epithelial cells

Sung Hwa Sohn, Jaebum Lee, Ki Nam Kim, In kyoung Kim, Meyoung Kon Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalEnvironmental Toxicology and Pharmacology
Issue number1
Publication statusPublished - 2009 Jan
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R13-2003-016-03002-0) and Korea Ministry of Environment as “The Eco-technopia 21 project (No. 2008-09002-0012-0)”.


  • 2-Naphthylamine
  • B(a)P
  • Gene expression
  • Microarray
  • Nicotine

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis


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