TY - JOUR
T1 - Effect of tobacco compounds on gene expression profiles in human epithelial cells
AU - Sohn, Sung Hwa
AU - Lee, Jaebum
AU - Kim, Ki Nam
AU - Kim, In kyoung
AU - Kim, Meyoung Kon
N1 - Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (No. R13-2003-016-03002-0) and Korea Ministry of Environment as “The Eco-technopia 21 project (No. 2008-09002-0012-0)”.
PY - 2009/1
Y1 - 2009/1
N2 - This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.
AB - This study was carried out to investigate the effects of the tobacco compounds (TC), nicotine, B(a)P, and 2-naphthylamine, on gene expression profiles in a human epithelial cells (A549). We treated A549 with the TC and analyzed gene expression using microarray and real-time PCR (RTP). Gene expression varied according to the TC used. By microarray, we found that apoptosis-related genes such as apoptosis-associated tyrosine kinase, interleukin 10 receptor beta, caspase 1 and DNA fragmentation factor beta subunit (40 kDa) were down-regulated in TC-treated A549 cells. RTP showed significant increases in the expression of Ahr, Arnt, CYP1A1, and CYP1B1 in TC-treated A549 cells. From these results, we suggest that tobacco compounds can influence apoptosis, inflammation, immunity, and the cell cycle in A549 cells. Also, our study demonstrates that a microarray-based genomic survey is a suitable high-throughput approach for the evaluation of gene expression and for the characterization of TC-induced toxicity.
KW - 2-Naphthylamine
KW - B(a)P
KW - Gene expression
KW - Microarray
KW - Nicotine
UR - http://www.scopus.com/inward/record.url?scp=57149145436&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2008.09.005
DO - 10.1016/j.etap.2008.09.005
M3 - Article
C2 - 21783928
AN - SCOPUS:57149145436
SN - 1382-6689
VL - 27
SP - 111
EP - 119
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 1
ER -