Effect of topical Na-hyaluronan on hemidesmosome formation in n-heptanol-induced corneal injury

Jang Hyun Chung; Won Ki Kim; Jin Sung Lee; Young Sook Pae; Hyung Jun Kim

doi:10.1159/000055460

Effect of topical Na-hyaluronan on hemidesmosome formation in n-heptanol-induced corneal injury

Jang Hyun Chung^*
, Won Ki Kim
, Jin Sung Lee
, Young Sook Pae
, Hyung Jun Kim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.

Original language	English
Pages (from-to)	96-100
Number of pages	5
Journal	Ophthalmic Research
Volume	30
Issue number	2
DOIs	https://doi.org/10.1159/000055460
Publication status	Published - 1998
Externally published	Yes

Keywords

Cornea
Hemidesmosomes
Na-hyaluronan
Rabbit
n-Heptanol

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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10.1159/000055460

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@article{0730fb2bf67040e88183869576aa0a13,

title = "Effect of topical Na-hyaluronan on hemidesmosome formation in n-heptanol-induced corneal injury",

abstract = "The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1\% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1\% Na-HA. The results suggest that topically applied 1\% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.",

keywords = "Cornea, Hemidesmosomes, Na-hyaluronan, Rabbit, n-Heptanol",

author = "Chung, \{Jang Hyun\} and Kim, \{Won Ki\} and Lee, \{Jin Sung\} and Pae, \{Young Sook\} and Kim, \{Hyung Jun\}",

year = "1998",

doi = "10.1159/000055460",

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volume = "30",

pages = "96--100",

journal = "Ophthalmic Research",

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TY - JOUR

T1 - Effect of topical Na-hyaluronan on hemidesmosome formation in n-heptanol-induced corneal injury

AU - Chung, Jang Hyun

AU - Kim, Won Ki

AU - Lee, Jin Sung

AU - Pae, Young Sook

AU - Kim, Hyung Jun

PY - 1998

Y1 - 1998

N2 - The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.

AB - The present study investigated the effect of Na-hyaluronan (Na-HA) on the hemidesmosome morphogenesis in n-heptanol-induced corneal wounds. Central epithelial wounds were induced in the rabbit cornea by applying a 5.5-mm round filter paper, which was soaked in n-heptanol, for 60 s. 1% Na-HA in phosphate-buffered saline (PBS) or PBS alone were topically administered to the wounded cornea 4 times daily for up to 7 days. Epithelial healing rates during the first 2 days were not altered by Na-HA. The number of hemidesmosomes in the basement membrane of the central cornea was significantly increased in both 3- and 7-day groups after treatment with 1% Na-HA. The results suggest that topically applied 1% Na-HA may enhance the formation of hemidesmosomes during the early healing phase in n-heptanol-induced corneal wounds.

KW - Cornea

KW - Hemidesmosomes

KW - Na-hyaluronan

KW - Rabbit

KW - n-Heptanol

UR - https://www.scopus.com/pages/publications/0031914834

U2 - 10.1159/000055460

DO - 10.1159/000055460

M3 - Article

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AN - SCOPUS:0031914834

SN - 0030-3747

VL - 30

SP - 96

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JO - Ophthalmic Research

JF - Ophthalmic Research

IS - 2

ER -

Effect of topical Na-hyaluronan on hemidesmosome formation in n-heptanol-induced corneal injury

Abstract

Keywords

ASJC Scopus subject areas

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