Abstract
Objectives: The aim of this study was to examine the cytotoxic effect and inflammatory response of human middle ear epithelial cells (HMEECs) induced by urban particles (UP). Materials and methods: Cell viability following UP exposure was assessed in HMEECs using the CCK 8 assay. The expression levels of the inflammation-related genes (COX-2 and MUC5AC) were analyzed using semi-quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and COX-2 production was analyzed using western blotting. Results: Treatment with UP decreased cell viability in HMEECs in a dose- and time-dependent manner. UP exposure induced the significantly increased expression of COX-2 and MUC5AC. Western blotting showed dose dependently increased expression of COX-2 production. Conclusions: UP decreased cell viability, increased the inflammatory response, and increased mucin gene production in HMEECs. These findings indicate that exposure to UP can contribute to the development of otitis media.
| Original language | English |
|---|---|
| Pages (from-to) | 777-781 |
| Number of pages | 5 |
| Journal | International Journal of Pediatric Otorhinolaryngology |
| Volume | 78 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2014 May |
Bibliographical note
Funding Information:This study was supported by the Korea Ministry of Environment as part of “ The Environmental Health Action Program ” ( 2012001360002 ).
Keywords
- Cyclooxygenase 2
- Human middle ear epithelial cells
- MUC5AC
- Otitis media
- Urban particles
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
- Otorhinolaryngology
