Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

Sun Uk Kim; Ying Hao Han; Tae Hoon Lee; Byung Hwa Hyun; Sang Ho Lee; Dong Seok Lee; Dae Yeul Yu

doi:10.1538/expanim.53.475

Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

Sun Uk Kim
, Ying Hao Han
, Tae Hoon Lee
, Byung Hwa Hyun
, Sang Ho Lee
, Dong Seok Lee
, Dae Yeul Yu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

Original language	English
Pages (from-to)	475-477
Number of pages	3
Journal	Experimental Animals
Volume	53
Issue number	5
DOIs	https://doi.org/10.1538/expanim.53.475
Publication status	Published - 2004

Keywords

C57BL/6 mice
Germ-line chimera
Host embryo

ASJC Scopus subject areas

Animal Science and Zoology
General Biochemistry,Genetics and Molecular Biology
General Veterinary

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10.1538/expanim.53.475

Cite this

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title = "Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells",

abstract = "Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.",

keywords = "C57BL/6 mice, Germ-line chimera, Host embryo",

author = "Kim, \{Sun Uk\} and Han, \{Ying Hao\} and Lee, \{Tae Hoon\} and Hyun, \{Byung Hwa\} and Lee, \{Sang Ho\} and Lee, \{Dong Seok\} and Yu, \{Dae Yeul\}",

year = "2004",

doi = "10.1538/expanim.53.475",

language = "English",

volume = "53",

pages = "475--477",

journal = "Experimental Animals",

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TY - JOUR

T1 - Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

AU - Kim, Sun Uk

AU - Han, Ying Hao

AU - Lee, Tae Hoon

AU - Hyun, Byung Hwa

AU - Lee, Sang Ho

AU - Lee, Dong Seok

AU - Yu, Dae Yeul

PY - 2004

Y1 - 2004

N2 - Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

AB - Blastocysts of C57BL/6 mice obtained either 2.5 or 3.5 days post-coitum (dpc) were examined for efficient microinjection after overnight in vitro culture. Incidences of zona-free embryos were much higher at 3.5 dpc after natural mating (1.05/mouse) and superovulation (2.83/mouse) than at 2.5 dpc after natural mating (0.05/mouse) and superovulation (0.01/mouse). By testing germ-line competency of gene-targeted J1 embryonic stem cells, superovulation and/or in vitro culture should be recommended for producing microinjectable blastocysts for production of high germ-line chimeras.

KW - C57BL/6 mice

KW - Germ-line chimera

KW - Host embryo

UR - https://www.scopus.com/pages/publications/16644377264

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DO - 10.1538/expanim.53.475

M3 - Article

C2 - 15516799

AN - SCOPUS:16644377264

SN - 1341-1357

VL - 53

SP - 475

EP - 477

JO - Experimental Animals

JF - Experimental Animals

IS - 5

ER -

Effective production of microinjectable blastocysts for germ-line transmission of embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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