Effects of carbon source and metabolic engineering on butyrate production in Escherichia coli

Sea Mi Joung, Nagendra Prasad Kurumbang, Byoung In Sang, Min Kyu Oh

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Butyrate was produced in recombinant Escherichia coli strains by applying metabolic engineering strategies. The genes for producing butyrate were cloned from Clostridium acetobutylicum and then expressed in E. coli. To study important factors for improving the productivity of butyrate, we deleted pta and ptsG genes in E. coli and compared the effects of these gene deletions in E. coli B and K strains. The effect of carbon sources, glucose and glycerol, was also compared. A significant improvement of butyrate production was made when glycerol was used as a carbon source, resulting in 0.56 g/l of butyrate in LB medium with 1% (v/v) glycerol.

    Original languageEnglish
    Pages (from-to)1587-1592
    Number of pages6
    JournalKorean Journal of Chemical Engineering
    Volume28
    Issue number7
    DOIs
    Publication statusPublished - 2011 Jul

    Keywords

    • Butyrate
    • Clostridium acetobutylicum
    • Escherichia coli
    • Metabolic Engineering

    ASJC Scopus subject areas

    • General Chemistry
    • General Chemical Engineering

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