TY - JOUR
T1 - Effects of carbon source and metabolic engineering on butyrate production in Escherichia coli
AU - Joung, Sea Mi
AU - Kurumbang, Nagendra Prasad
AU - Sang, Byoung In
AU - Oh, Min Kyu
PY - 2011/7
Y1 - 2011/7
N2 - Butyrate was produced in recombinant Escherichia coli strains by applying metabolic engineering strategies. The genes for producing butyrate were cloned from Clostridium acetobutylicum and then expressed in E. coli. To study important factors for improving the productivity of butyrate, we deleted pta and ptsG genes in E. coli and compared the effects of these gene deletions in E. coli B and K strains. The effect of carbon sources, glucose and glycerol, was also compared. A significant improvement of butyrate production was made when glycerol was used as a carbon source, resulting in 0.56 g/l of butyrate in LB medium with 1% (v/v) glycerol.
AB - Butyrate was produced in recombinant Escherichia coli strains by applying metabolic engineering strategies. The genes for producing butyrate were cloned from Clostridium acetobutylicum and then expressed in E. coli. To study important factors for improving the productivity of butyrate, we deleted pta and ptsG genes in E. coli and compared the effects of these gene deletions in E. coli B and K strains. The effect of carbon sources, glucose and glycerol, was also compared. A significant improvement of butyrate production was made when glycerol was used as a carbon source, resulting in 0.56 g/l of butyrate in LB medium with 1% (v/v) glycerol.
KW - Butyrate
KW - Clostridium acetobutylicum
KW - Escherichia coli
KW - Metabolic Engineering
UR - http://www.scopus.com/inward/record.url?scp=79959793359&partnerID=8YFLogxK
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U2 - 10.1007/s11814-011-0032-6
DO - 10.1007/s11814-011-0032-6
M3 - Article
AN - SCOPUS:79959793359
SN - 0256-1115
VL - 28
SP - 1587
EP - 1592
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
IS - 7
ER -