EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE

Jae W. Park; T. C. LANIER; H. E. SWAISGOOD; D. D. HAMANN; J. T. KEETON

doi:10.1111/j.1745-4514.1987.tb00119.x

EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE

Jae W. Park, T. C. LANIER, H. E. SWAISGOOD, D. D. HAMANN, J. T. KEETON

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Physicochemical changes in bovine natural actomyosin extracted from prerigor semimembranosus muscle were investigated during frozen storage at −28°C as affected by the addition of cryoprotectants (5.6% Polydextrose® or 5.6% mixture (1:1) of sucrose and sorbitol). Proteins were destabilized during freezing and frozen storage as reflected by decreases in protein solubility, the visual appearance of aggregates in protein sols, decrease in intensity of flow birefringence, intrinsic viscosity and ATPase activity, and changes in size, shape, or charge of the protein (especially myosin) as evidenced by nondenaturing electrophoresis. These effects were reduced to some extent by the two cryoprotectant treatments.

Original language	English
Pages (from-to)	143-161
Number of pages	19
Journal	Journal of Food Biochemistry
Volume	11
Issue number	2
DOIs	https://doi.org/10.1111/j.1745-4514.1987.tb00119.x
Publication status	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Food Science
Biophysics
Pharmacology
Cell Biology

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10.1111/j.1745-4514.1987.tb00119.x

Cite this

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title = "EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE",

abstract = "Physicochemical changes in bovine natural actomyosin extracted from prerigor semimembranosus muscle were investigated during frozen storage at −28°C as affected by the addition of cryoprotectants (5.6% Polydextrose{\textregistered} or 5.6% mixture (1:1) of sucrose and sorbitol). Proteins were destabilized during freezing and frozen storage as reflected by decreases in protein solubility, the visual appearance of aggregates in protein sols, decrease in intensity of flow birefringence, intrinsic viscosity and ATPase activity, and changes in size, shape, or charge of the protein (especially myosin) as evidenced by nondenaturing electrophoresis. These effects were reduced to some extent by the two cryoprotectant treatments.",

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doi = "10.1111/j.1745-4514.1987.tb00119.x",

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AU - Park, Jae W.

AU - LANIER, T. C.

AU - SWAISGOOD, H. E.

AU - HAMANN, D. D.

AU - KEETON, J. T.

PY - 1987

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AB - Physicochemical changes in bovine natural actomyosin extracted from prerigor semimembranosus muscle were investigated during frozen storage at −28°C as affected by the addition of cryoprotectants (5.6% Polydextrose® or 5.6% mixture (1:1) of sucrose and sorbitol). Proteins were destabilized during freezing and frozen storage as reflected by decreases in protein solubility, the visual appearance of aggregates in protein sols, decrease in intensity of flow birefringence, intrinsic viscosity and ATPase activity, and changes in size, shape, or charge of the protein (especially myosin) as evidenced by nondenaturing electrophoresis. These effects were reduced to some extent by the two cryoprotectant treatments.

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EFFECTS OF CRYOPROTECTANTS IN MINIMIZING PHYSICOCHEMICAL CHANGES OF BOVINE NATURAL ACTOMYOSIN DURING FROZEN STORAGE

Abstract

ASJC Scopus subject areas

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