Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells

Sun Ho Kee; Eun Jin Cho; Jin Won Song; Kwang Sook Park; Luck Ju Baek; Ki Joon Song

doi:10.1111/j.1348-0421.2004.tb03614.x

Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells

Sun Ho Kee, Eun Jin Cho, Jin Won Song, Kwang Sook Park, Luck Ju Baek, Ki Joon Song

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

It has been suggested that infectious entry of rubella virus (RV) is conducted by receptor mediated endocytosis. To explore the cellular entry mechanism of RV, inhibitory effects of drugs affecting various endocytic pathways on RV entry into VeroE6 cells were analyzed. Results showed that RV infectious entry into VeroE6 cells is mediated by clathrin-dependent endocytosis and not by caveolae-mediated endocytosis. Moreover, chemical inhibition of macropinocytosis such as treatments of amiloride, actin and microtubule- disrupting drug significantly reduced RV infection. Considering that macropinocytosis is inducible endocytosis by cellular stimulations, clathrin-mediated endocytosis is likely to be a major route of RV infectious entry.

Original language	English
Pages (from-to)	823-829
Number of pages	7
Journal	Microbiology and Immunology
Volume	48
Issue number	11
DOIs	https://doi.org/10.1111/j.1348-0421.2004.tb03614.x
Publication status	Published - 2004

Keywords

Clathrin
Endocytosis
Macropinocytosis
Rubella virus

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.2004.tb03614.x

Cite this

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title = "Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells",

abstract = "It has been suggested that infectious entry of rubella virus (RV) is conducted by receptor mediated endocytosis. To explore the cellular entry mechanism of RV, inhibitory effects of drugs affecting various endocytic pathways on RV entry into VeroE6 cells were analyzed. Results showed that RV infectious entry into VeroE6 cells is mediated by clathrin-dependent endocytosis and not by caveolae-mediated endocytosis. Moreover, chemical inhibition of macropinocytosis such as treatments of amiloride, actin and microtubule- disrupting drug significantly reduced RV infection. Considering that macropinocytosis is inducible endocytosis by cellular stimulations, clathrin-mediated endocytosis is likely to be a major route of RV infectious entry.",

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T1 - Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells

AU - Kee, Sun Ho

AU - Cho, Eun Jin

AU - Song, Jin Won

AU - Park, Kwang Sook

AU - Baek, Luck Ju

AU - Song, Ki Joon

PY - 2004

Y1 - 2004

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AB - It has been suggested that infectious entry of rubella virus (RV) is conducted by receptor mediated endocytosis. To explore the cellular entry mechanism of RV, inhibitory effects of drugs affecting various endocytic pathways on RV entry into VeroE6 cells were analyzed. Results showed that RV infectious entry into VeroE6 cells is mediated by clathrin-dependent endocytosis and not by caveolae-mediated endocytosis. Moreover, chemical inhibition of macropinocytosis such as treatments of amiloride, actin and microtubule- disrupting drug significantly reduced RV infection. Considering that macropinocytosis is inducible endocytosis by cellular stimulations, clathrin-mediated endocytosis is likely to be a major route of RV infectious entry.

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KW - Macropinocytosis

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Effects of endocytosis inhibitory drugs on rubella virus entry into VeroE6 cells

Abstract

Keywords

ASJC Scopus subject areas

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