Effects of fermented milk with mixed strains as a probiotic on the inhibition of loperamide-induced constipation

Byoung Kook Kim, In Suk Choi, Jihee Kim, Sung Hee Han, Hyung Joo Suh, Jae Kwan Hwang

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6 Citations (Scopus)


To investigate the effects of a single bacterium and a mixture of bacteria as probiotics in loperamide- treated animal models, loperamide (3 mg/kg) was administered to SD rats to induce constipation. The individual lactic acid bacterial doses, Enterococcus faecium (EF), Lactobacillus acidophilus (LA), Streptococcus thermophilus (ST), Bifidobacterium bifidum (BB), Bifidobacterium lactis (BL), Pediococcus pentosaceus (PP), and a mixture of the bacteria were orally administered to loperamide-induced constipated rats at a concentration of 108 CFU/kg for 14 days. The weights and water contents of their stools were found to be significantly higher in PP, CKDB (mixture of 5 strains except PP), and CKDBP (CKDB+PP) groups than in the normal (constipation not induced) and the control (constipation-induced) groups (p<0.05). The intestinal transit ratio was significantly higher in all probiotic-treated groups than in the control group, and was the highest in the CKDBP group (p<0.05). The mucosal length and mucus secretion were significantly improved in all probiotic-treated-groups, as compared to that in the control group, and the CKDBP group was found to be the most effective according to immunohistochemistry (IHC) staining and total short chain fatty acid content analysis (p<0.05). Lastly, PP, CKDB, and CKDBP showed relatively higher Lactobacillus sp. ratios of 61.94%, 60.31% and 51.94%, respectively, compared to the other groups, based on metagenomic analysis.

Original languageEnglish
Pages (from-to)906-916
Number of pages11
JournalKorean Journal for Food Science of Animal Resources
Issue number6
Publication statusPublished - 2017 Dec

Bibliographical note

Publisher Copyright:
© 2017 Korean Society for Food Science of Animal Resources.


  • Bacteria
  • Constipation
  • Loperamide
  • Probiotic

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology


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