Effects of immobilizations of rhBMP-2 and/or rhPDGF-BB on titanium implant surfaces on osseointegration and bone regeneration

So Hyoun Lee, Eun Bin Bae, Sung Eun Kim, Young Pil Yun, Hak Jun Kim, Jae Won Choi, Jin Ju Lee, Jung Bo Huh

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)


    The aim of this study was to examine the effects of immobilizing rhPDGF-BB plus rhBMP-2 on heparinized-Ti implants on in vivo osseointegration and vertical bone regeneration at alveolar ridges. Successful immobilizations of rhPDGF-BB and/or rhBMP-2 onto heparinized-Ti (Hepa/Ti) were confirmed by in vitro analysis, and both growth factors were found to be sustained release. To evaluate bone regeneration, rhPDGF-BB, and/or rhBMP-2-immobilized Hepa/Ti implants were inserted into beagle dogs; implant stability quotients (ISQ), bone mineral densities, bone volumes, osseointegration, and bone formation were assessed by micro CT and histometrically. In vivo study showed that the osseointegration and bone formation were greater in the rhPDGF-BB/rhBMP-2-immobilized Hepa/Ti group than in the rhPDGF-BB-immobilized Hepa/Ti group. The rhPDGF-BB/rhBMP-2 immobilized Hepa/Ti group also showed better implant stability and greater bone volume around defect areas and intra-thread bone density (ITBD) than the rhBMP-2-immobilized Hepa/Ti group. However, no significant differences were observed between these two groups. Through these results, we conclude rhBMP-2 immobilized, heparin-grafted implants appear to offer a suitable delivery system that enhances new bone formation in defect areas around implants. However, we failed to observe the synergetic effects for the rhBMP-2 and rhPDGF-BB combination.

    Original languageEnglish
    Article number17
    Issue number1
    Publication statusPublished - 2018 Jan 1

    Bibliographical note

    Funding Information:
    Acknowledgments: This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2017R1A2B4005820).

    Publisher Copyright:
    © 2018 by the authors.


    • Beagle dog
    • Bone regeneration
    • Heparin
    • Implant surface
    • Osseointegration
    • RhBMP-2
    • RhPDGF-BB

    ASJC Scopus subject areas

    • Surfaces and Interfaces
    • Surfaces, Coatings and Films
    • Materials Chemistry


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