Effects of ionic liquid mixtures on lipid extraction from Chlorella vulgaris

Sun A. Choi, You Kwan Oh, Min Ji Jeong, Seung Wook Kim, Jin Suk Lee, Ji Yeon Park

Research output: Contribution to journalArticlepeer-review

111 Citations (Scopus)


In this study, pre-biodiesel-production lipid extraction from microalgae (Chlorella vulgaris) was performed. The yield-enhancing effects of ionic liquid blends on the lipid extraction were investigated. The initial fatty acids content of the C.vulgaris was 292.2mg/g cell. The lipid extraction yield using single ionic liquids was compared with the yield obtained with organic solvents and ionic liquid mixtures, respectively. The yield by hexane-methanol solvent was 185.4mg/g cell. Among the 12 ionic liquids, 1-ethyl-3-methyl imidazolium acetate, 1-ethyl-3-methyl imidazolium diethylphosphate, 1-ethyl-3-methyl imidazolium tetrafluoroborate, and 1-ethyl-3-methyl imidazolium chloride showed high (>200.0mg/gcell) lipid extraction yields. Although the yields of 1-ethyl-3-methyl imidazolium ethyl sulfate and 1-ethyl-3-methyl imidazolium thiocyanate were only 60.5 and 42.7mg/g cell, respectively, the yield for their mixture (weight ratio of 1:1) was improved to 158.2mg/g cell. Similarly, whereas the lipid extraction yield of 1-ethyl-3-methyl imidazolium hydrogen sulfate was just 35.2mg/g cell, that for its mixture with 1-ethyl-3-methyl imidazolium thiocyanate (weight ratio of 1:1) was boosted to 200.6mg/g cell. Overall, the synergistic effects of the ionic liquid mixtures with different anions improved the lipid extraction yield of C.vulgaris.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalRenewable Energy
Publication statusPublished - 2014 May


  • 1-Ethyl-3-methyl imidazolium cation
  • Chlorella vulgaris
  • Ionic liquid mixtures
  • Lipid extraction
  • Microalgal oil

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment


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