Effects of lipopolysaccharide on changes in red blood cells in a mice endotoxemia model

Jeihak Myung, Seol Ju Park, Jaekwan Lim, Yun Hee Kim, Sehyun Shin, Choon Hak Lim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The aim of this study was to evaluate changes in RBC aggregation and deformability over 24 hr and suggest specific shear stress values for detecting RBC deformability in a mouse endotoxemia model using lipopolysaccharide (LPS). Six-week-old male BALB/c mice received LPS (20 mg/kg) intraperitoneally. Aggregation indices (AIs) and T1/2 were measured to assess RBC aggregation, and elongation indices (EIs) were used to assess RBC deformability at shear stress values of 0.3, 0.5, 1, 3, 7, 10, 15 and 20 Pascals (Pa) 0, 30 min, 1, 2, 4, 6, 9, 12, 18 and 24 hours after the LPS injection. No significant differences were detected in the AIs during the study period, however, T1/2 shortened significantly 2, 6, 12, 18, and 24 hr after the LPS injection. The EIs increased significantly 24 hr after LPS injection at 0.5 and 1 Pa shear stress, whereas it decreased significantly at 10 Pa of shear stress 24 hr after the LPS injection. Altered RBC deformability was detectable 24 hr after the LPS injection and T1/2 may be a sensitive marker for detecting changes in RBC aggregation. The EIs should be measured at 1 Pa to detect changes in RBC deformability in LPS-induced septic mice.

Original languageEnglish
Pages (from-to)305-312
Number of pages8
JournalClinical Hemorheology and Microcirculation
Issue number4
Publication statusPublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 - IOS Press and the authors. All rights reserved.

ASJC Scopus subject areas

  • Physiology
  • Hematology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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