Effects of nutrients and culture conditions on morphology in the seed culture of Cephalosporium acremonium ATCC 20339

Myung Sun Lee; Jung Soo Lim; Chang Ho Kim; Kyung Keun Oh; Suk In Hong; Seung Wook Kim

doi:10.1007/BF02931963

Effects of nutrients and culture conditions on morphology in the seed culture of Cephalosporium acremonium ATCC 20339

Myung Sun Lee, Jung Soo Lim, Chang Ho Kim, Kyung Keun Oh, Suk In Hong, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The objective of this study was to investigate the effects of nutrients and culture conditions on morphology during the seed culture of C. acremonium ATCC 20339. Morphological factors such as hyphal length, number of tips, number of arthrospores were observed to investigate the relationship between seed morphology and CPC production. During the time course of seed culture, hyphal length was shortened and the number of arthrospores increased rapidly. On the other hand, the number of tips decreased rapidly, and this was closely related to the hyphal length. Mixed nitrogen sources of 3% soybean meal and 1% cotton seed flour were determined as the proper organic nitrogen sources, in terms of the morphological factors in the seed culture. This fact was proven in batch culture for the production of Cephalosporin C. It was also found that a proper agitation speed enhanced the morphological differentiation of C. acremonium ATCC 20339, thus improving the production of Cephalosporin C.

Original language	English
Pages (from-to)	156-160
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	6
Issue number	2
DOIs	https://doi.org/10.1007/BF02931963
Publication status	Published - 2001

Bibliographical note

Funding Information:
Aknowledgements This study was partially supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) ar Korea University, Seoul, Korea.

Keywords

Cephalosporin C
Cephalosporium acremonium
Image analysis
Morphology

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/BF02931963

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title = "Effects of nutrients and culture conditions on morphology in the seed culture of Cephalosporium acremonium ATCC 20339",

abstract = "The objective of this study was to investigate the effects of nutrients and culture conditions on morphology during the seed culture of C. acremonium ATCC 20339. Morphological factors such as hyphal length, number of tips, number of arthrospores were observed to investigate the relationship between seed morphology and CPC production. During the time course of seed culture, hyphal length was shortened and the number of arthrospores increased rapidly. On the other hand, the number of tips decreased rapidly, and this was closely related to the hyphal length. Mixed nitrogen sources of 3% soybean meal and 1% cotton seed flour were determined as the proper organic nitrogen sources, in terms of the morphological factors in the seed culture. This fact was proven in batch culture for the production of Cephalosporin C. It was also found that a proper agitation speed enhanced the morphological differentiation of C. acremonium ATCC 20339, thus improving the production of Cephalosporin C.",

keywords = "Cephalosporin C, Cephalosporium acremonium, Image analysis, Morphology",

author = "Lee, {Myung Sun} and Lim, {Jung Soo} and Kim, {Chang Ho} and Oh, {Kyung Keun} and Hong, {Suk In} and Kim, {Seung Wook}",

note = "Funding Information: Aknowledgements This study was partially supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) ar Korea University, Seoul, Korea.",

year = "2001",

doi = "10.1007/BF02931963",

language = "English",

volume = "6",

pages = "156--160",

journal = "Biotechnology and Bioprocess Engineering",

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T1 - Effects of nutrients and culture conditions on morphology in the seed culture of Cephalosporium acremonium ATCC 20339

AU - Lee, Myung Sun

AU - Lim, Jung Soo

AU - Kim, Chang Ho

AU - Oh, Kyung Keun

AU - Hong, Suk In

AU - Kim, Seung Wook

N1 - Funding Information: Aknowledgements This study was partially supported by research grants from the Korea Science and Engineering Foundation (KOSEF) through the Applied Rheology Center (ARC), an official KOSEF-created engineering research center (ERC) ar Korea University, Seoul, Korea.

PY - 2001

Y1 - 2001

N2 - The objective of this study was to investigate the effects of nutrients and culture conditions on morphology during the seed culture of C. acremonium ATCC 20339. Morphological factors such as hyphal length, number of tips, number of arthrospores were observed to investigate the relationship between seed morphology and CPC production. During the time course of seed culture, hyphal length was shortened and the number of arthrospores increased rapidly. On the other hand, the number of tips decreased rapidly, and this was closely related to the hyphal length. Mixed nitrogen sources of 3% soybean meal and 1% cotton seed flour were determined as the proper organic nitrogen sources, in terms of the morphological factors in the seed culture. This fact was proven in batch culture for the production of Cephalosporin C. It was also found that a proper agitation speed enhanced the morphological differentiation of C. acremonium ATCC 20339, thus improving the production of Cephalosporin C.

AB - The objective of this study was to investigate the effects of nutrients and culture conditions on morphology during the seed culture of C. acremonium ATCC 20339. Morphological factors such as hyphal length, number of tips, number of arthrospores were observed to investigate the relationship between seed morphology and CPC production. During the time course of seed culture, hyphal length was shortened and the number of arthrospores increased rapidly. On the other hand, the number of tips decreased rapidly, and this was closely related to the hyphal length. Mixed nitrogen sources of 3% soybean meal and 1% cotton seed flour were determined as the proper organic nitrogen sources, in terms of the morphological factors in the seed culture. This fact was proven in batch culture for the production of Cephalosporin C. It was also found that a proper agitation speed enhanced the morphological differentiation of C. acremonium ATCC 20339, thus improving the production of Cephalosporin C.

KW - Cephalosporin C

KW - Cephalosporium acremonium

KW - Image analysis

KW - Morphology

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SN - 1226-8372

VL - 6

SP - 156

EP - 160

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 2

ER -

Effects of nutrients and culture conditions on morphology in the seed culture of Cephalosporium acremonium ATCC 20339

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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