TY - JOUR
T1 - Effects of porcine testis extract on wound healing in rat
AU - Lee, Dong Mok
AU - Bhat, Abdul Roouf
AU - Kim, Yong Woon
AU - Shin, Dong Hoon
AU - Kim, Joo Young
AU - Kim, Keuk Jun
AU - Lee, Ki Ho
AU - Cheon, Yong Pil
AU - Chun, Taehoon
AU - Choi, Inho
N1 - Funding Information:
This study was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forest and Fisheries), Ministry for Food, Agriculture, Forest and Fisheries, Republic of Korea.
PY - 2012/12
Y1 - 2012/12
N2 - Sex hormones have long been considered to play an important role in bone turnover rate, periodontal diseases, and wound healing. We have studied the effect of porcine testis steroid extract (PTSE), an extract of porcine testes, which holds a good ratio of 19-nortestosterone (nandrolone), testosterone, androstenedione, 17β-estradiol, and estrone, on the healing rate of a standardized full-thickness linear wound on the back of the rat. Skin punch or carbon dioxide (CO2) laser methods were used to create the deep skin injury in two groups of animals. The animals were treated with the PTSE cream, control cream and Vaseline (control) to find out the effect in re-epithelialization, contraction, and formation of granulation and scar tissues. Histological examination after 21 days showed 100, 87.4, and 80.5% recovery of epidermis, dermis, and hypodermis, respectively in the PTSE-treated animals. Similarly, on the 15th day of treatment, complete healing of intact skin was observed in the PTSE cream-treated animals among the laser radiation group. Even though the beginning of re-epithelialization phase and completion of serum crust formation was also observed in the base cream- and Vaseline-treated animals respectively, the complete healing cycle was observed only in the PTSE-treated group. The white blood cell count in the PTSE-treated group showed that PTSE cream is nontoxic to animals.
AB - Sex hormones have long been considered to play an important role in bone turnover rate, periodontal diseases, and wound healing. We have studied the effect of porcine testis steroid extract (PTSE), an extract of porcine testes, which holds a good ratio of 19-nortestosterone (nandrolone), testosterone, androstenedione, 17β-estradiol, and estrone, on the healing rate of a standardized full-thickness linear wound on the back of the rat. Skin punch or carbon dioxide (CO2) laser methods were used to create the deep skin injury in two groups of animals. The animals were treated with the PTSE cream, control cream and Vaseline (control) to find out the effect in re-epithelialization, contraction, and formation of granulation and scar tissues. Histological examination after 21 days showed 100, 87.4, and 80.5% recovery of epidermis, dermis, and hypodermis, respectively in the PTSE-treated animals. Similarly, on the 15th day of treatment, complete healing of intact skin was observed in the PTSE cream-treated animals among the laser radiation group. Even though the beginning of re-epithelialization phase and completion of serum crust formation was also observed in the base cream- and Vaseline-treated animals respectively, the complete healing cycle was observed only in the PTSE-treated group. The white blood cell count in the PTSE-treated group showed that PTSE cream is nontoxic to animals.
KW - PTSE
KW - collagen
KW - skin injuries
KW - wound healing
UR - http://www.scopus.com/inward/record.url?scp=84870890863&partnerID=8YFLogxK
U2 - 10.1080/19768354.2012.726645
DO - 10.1080/19768354.2012.726645
M3 - Article
AN - SCOPUS:84870890863
SN - 1976-8354
VL - 16
SP - 469
EP - 478
JO - Animal Cells and Systems
JF - Animal Cells and Systems
IS - 6
ER -