Effects of vibration on the proteome expression of anterior cruciate ligament cells

Gi Yeon Han, Seung Ah Park, Ji Hye Kim, Eun Kyung Lee, Hyun Jung Kim, Young Kwon Seo, Jung Keug Park, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Recent reports have suggested that vibration has beneficial effects on knee healing response; however, the biomechanism of these beneficial effects still need to be determined on the anterior cruciate ligament (ACL) cell level. In this study, we applied a 20 Hz vibration to ACL cells, which produced a 20% increase (P< 0.001) in cell activity and 17% increase (P< 0.001) in intracellular sulfated glycosaminoglycan levels. In the 20 Hz vibration-stimulated ACL cell group, eight up-regulated (100 ~ 300%) protein spots were identified compared with the control group by proteomics analysis. Among these proteins, Annexin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71% and 16% higher expression, respectively, in the 20 Hz vibration-stimulated ACL cell group by Western blotting (P< 0.001). These results indicate that vibration produces a positive cellular environment, and Annexin A2 and prolyl 4 hydroxylase are expected to help ligament repair and ACL cell proliferation by controlling cell membrane and extracellular matrix formation.

Original languageEnglish
Pages (from-to)783-789
Number of pages7
JournalExperimental Biology and Medicine
Issue number7
Publication statusPublished - 2011 Jul


  • Annexin A2
  • Anterior cruciate ligament
  • Extracellular matrix
  • Prolyl 4 hydroxylase
  • Vibration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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