Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel

Eunji Kim; Yoon Seok Song; Han Suk Choi; Hah Young Yoo; Seong Woo Kang; Kwang Ho Song; Sung Ok Han; Seung Wook Kim

doi:10.1007/s12257-011-0085-8

Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel

Eunji Kim, Yoon Seok Song, Han Suk Choi, Hah Young Yoo, Seong Woo Kang, Kwang Ho Song, Sung Ok Han, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.

Original language	English
Pages (from-to)	55-59
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	17
Issue number	1
DOIs	https://doi.org/10.1007/s12257-011-0085-8
Publication status	Published - 2012 Feb

Keywords

Cellobiose dehydrogenase (CDH)
Immobilization
Phanerochaete chrysosporium
Pretreatment
White-rot fungi

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Biomedical Engineering

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10.1007/s12257-011-0085-8

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title = "Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel",

abstract = "In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.",

keywords = "Cellobiose dehydrogenase (CDH), Immobilization, Phanerochaete chrysosporium, Pretreatment, White-rot fungi",

author = "Eunji Kim and Song, {Yoon Seok} and Choi, {Han Suk} and Yoo, {Hah Young} and Kang, {Seong Woo} and Song, {Kwang Ho} and Han, {Sung Ok} and Kim, {Seung Wook}",

note = "Funding Information: This research was supported by the Technology Development Program (309016-5) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (2009-0073856). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.",

year = "2012",

month = feb,

doi = "10.1007/s12257-011-0085-8",

language = "English",

volume = "17",

pages = "55--59",

journal = "Biotechnology and Bioprocess Engineering",

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publisher = "Korean Society for Biotechnology and Bioengineering",

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AU - Kim, Eunji

AU - Song, Yoon Seok

AU - Choi, Han Suk

AU - Yoo, Hah Young

AU - Kang, Seong Woo

AU - Song, Kwang Ho

AU - Han, Sung Ok

AU - Kim, Seung Wook

N1 - Funding Information: This research was supported by the Technology Development Program (309016-5) for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea and the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (2009-0073856). Copyright: Copyright 2013 Elsevier B.V., All rights reserved.

PY - 2012/2

Y1 - 2012/2

N2 - In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.

AB - In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.

KW - Cellobiose dehydrogenase (CDH)

KW - Immobilization

KW - Phanerochaete chrysosporium

KW - Pretreatment

KW - White-rot fungi

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Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel

Abstract

Keywords

ASJC Scopus subject areas

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