Efficient induction of antigen-specific, T helper type 1-mediated immune responses by intramuscular injection with ovalbumin/interleukin-18 fusion DNA

Seung H. Kim; Daeho Cho; Seung Y. Hwang; Tae S. Kim

doi:10.1016/S0264-410X(01)00175-X

Efficient induction of antigen-specific, T helper type 1-mediated immune responses by intramuscular injection with ovalbumin/interleukin-18 fusion DNA

Seung H. Kim, Daeho Cho, Seung Y. Hwang, Tae S. Kim

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The balance of two types of T helper (Th) cells, Th1 and Th2 subsets, is important with respect to susceptibility or resistance to particular infections, or to autoimmune diseases and allergic diseases. To effectively induce Th1 immune responses in an antigen-specific manner, we constructed a mammalian expression plasmid (pOVA/IL-18) carrying a fusion gene in which the ovalbumin (OVA; a model antigen) cDNA was covalently linked to mature interleukin-18 (IL-18) cDNA. Intramuscular injection of C57BL/6 mice with the pOVA/IL-18 DNA efficiently increased the production of both OVA-specific IFN-γ and anti-OVA IgG2a isotype, compared with the injection with the pOVA DNA. In addition, the pOVA/IL-18 was more efficient than a simple mixture of pOVA and pIL-18 in inducing antigen-specific, Th1 immune responses and in inhibiting OVA-specific, IL-4 production. These studies indicate that vaccination with the OVA/IL-18 fusion DNA efficiently induces Th1 immune response in an antigen-specific manner.

Original language	English
Pages (from-to)	4107-4114
Number of pages	8
Journal	Vaccine
Volume	19
Issue number	30
DOIs	https://doi.org/10.1016/S0264-410X(01)00175-X
Publication status	Published - 2001 Jul 20
Externally published	Yes

Keywords

DNA vaccine
Interleukin-18
Ovalbumin
T helper response

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(01)00175-X

Cite this

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title = "Efficient induction of antigen-specific, T helper type 1-mediated immune responses by intramuscular injection with ovalbumin/interleukin-18 fusion DNA",

abstract = "The balance of two types of T helper (Th) cells, Th1 and Th2 subsets, is important with respect to susceptibility or resistance to particular infections, or to autoimmune diseases and allergic diseases. To effectively induce Th1 immune responses in an antigen-specific manner, we constructed a mammalian expression plasmid (pOVA/IL-18) carrying a fusion gene in which the ovalbumin (OVA; a model antigen) cDNA was covalently linked to mature interleukin-18 (IL-18) cDNA. Intramuscular injection of C57BL/6 mice with the pOVA/IL-18 DNA efficiently increased the production of both OVA-specific IFN-γ and anti-OVA IgG2a isotype, compared with the injection with the pOVA DNA. In addition, the pOVA/IL-18 was more efficient than a simple mixture of pOVA and pIL-18 in inducing antigen-specific, Th1 immune responses and in inhibiting OVA-specific, IL-4 production. These studies indicate that vaccination with the OVA/IL-18 fusion DNA efficiently induces Th1 immune response in an antigen-specific manner.",

keywords = "DNA vaccine, Interleukin-18, Ovalbumin, T helper response",

author = "Kim, {Seung H.} and Daeho Cho and Hwang, {Seung Y.} and Kim, {Tae S.}",

note = "Funding Information: We thank Drs M.E. Reff and D.S. Lim for providing valuable reagents, and Drs S.H. Choi and J.W. Lee for helpful discussion. We also wish to thank E.J. Kim for technical assistance. This work was supported by grants from the KOSEF (HRC 1998G0201) and from the GenoCheck Co., Ltd.",

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doi = "10.1016/S0264-410X(01)00175-X",

language = "English",

volume = "19",

pages = "4107--4114",

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TY - JOUR

T1 - Efficient induction of antigen-specific, T helper type 1-mediated immune responses by intramuscular injection with ovalbumin/interleukin-18 fusion DNA

AU - Kim, Seung H.

AU - Cho, Daeho

AU - Hwang, Seung Y.

AU - Kim, Tae S.

N1 - Funding Information: We thank Drs M.E. Reff and D.S. Lim for providing valuable reagents, and Drs S.H. Choi and J.W. Lee for helpful discussion. We also wish to thank E.J. Kim for technical assistance. This work was supported by grants from the KOSEF (HRC 1998G0201) and from the GenoCheck Co., Ltd.

PY - 2001/7/20

Y1 - 2001/7/20

N2 - The balance of two types of T helper (Th) cells, Th1 and Th2 subsets, is important with respect to susceptibility or resistance to particular infections, or to autoimmune diseases and allergic diseases. To effectively induce Th1 immune responses in an antigen-specific manner, we constructed a mammalian expression plasmid (pOVA/IL-18) carrying a fusion gene in which the ovalbumin (OVA; a model antigen) cDNA was covalently linked to mature interleukin-18 (IL-18) cDNA. Intramuscular injection of C57BL/6 mice with the pOVA/IL-18 DNA efficiently increased the production of both OVA-specific IFN-γ and anti-OVA IgG2a isotype, compared with the injection with the pOVA DNA. In addition, the pOVA/IL-18 was more efficient than a simple mixture of pOVA and pIL-18 in inducing antigen-specific, Th1 immune responses and in inhibiting OVA-specific, IL-4 production. These studies indicate that vaccination with the OVA/IL-18 fusion DNA efficiently induces Th1 immune response in an antigen-specific manner.

AB - The balance of two types of T helper (Th) cells, Th1 and Th2 subsets, is important with respect to susceptibility or resistance to particular infections, or to autoimmune diseases and allergic diseases. To effectively induce Th1 immune responses in an antigen-specific manner, we constructed a mammalian expression plasmid (pOVA/IL-18) carrying a fusion gene in which the ovalbumin (OVA; a model antigen) cDNA was covalently linked to mature interleukin-18 (IL-18) cDNA. Intramuscular injection of C57BL/6 mice with the pOVA/IL-18 DNA efficiently increased the production of both OVA-specific IFN-γ and anti-OVA IgG2a isotype, compared with the injection with the pOVA DNA. In addition, the pOVA/IL-18 was more efficient than a simple mixture of pOVA and pIL-18 in inducing antigen-specific, Th1 immune responses and in inhibiting OVA-specific, IL-4 production. These studies indicate that vaccination with the OVA/IL-18 fusion DNA efficiently induces Th1 immune response in an antigen-specific manner.

KW - DNA vaccine

KW - Interleukin-18

KW - Ovalbumin

KW - T helper response

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AN - SCOPUS:0035919526

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ER -

Efficient induction of antigen-specific, T helper type 1-mediated immune responses by intramuscular injection with ovalbumin/interleukin-18 fusion DNA

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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