Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA

Keyphrases

• Targeting Efficiency 100%
• Guide RNA (gRNA) 100%
• CRISPR Interference (CRISPRi) 100%
• Ablation 30%
• Screening Data 20%
• Vemurafenib 20%
• Malignant Cells 10%
• In Cancer 10%
• Novel Therapeutic Targets 10%
• A375 Cells 10%
• Novel Genes 10%
• Human Melanoma Cells 10%
• Single Guide RNA (sgRNA) 10%
• Synergize 10%
• Drug Discovery 10%
• Low Activity 10%
• Change of Variables 10%
• Indel 10%
• PTBP1 10%
• Power Generation Efficiency 10%
• Activity Data 10%
• SgRNA Library 10%
• Indel mutation 10%
• False-negative Results 10%
• Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) 10%
• Target Cleavage 10%
• New Analysis Method 10%
• CRISPR-Cas9 Screen 10%
• Nicotinamide N-methyltransferase 10%
• Synergistic Targets 10%
• CRISPR Screening 10%
• Lactate Dehydrogenase B 10%
• Cleavage Efficiency 10%
• Fold Change Analysis 10%

Biochemistry, Genetics and Molecular Biology

• CRISPR 100%
• Guide RNA 100%
• Indel 50%
• Vemurafenib 50%
• Binding Protein 25%
• Synapsin I 25%
• Essential Gene 25%
• Lactate Dehydrogenase 25%
• Polypyrimidine Tract 25%
• CRISPR/Cas9 25%
• Nicotinamide N-Methyltransferase 25%