Efficient succinic acid production using a biochar-treated textile waste hydrolysate in an in situ fibrous bed bioreactor

Xiaotong Li, Ming Zhang, Jiewen Luo, Shicheng Zhang, Xiao Yang, Avanthi Deshani Igalavithana, Yong Sik Ok, Daniel C.W. Tsang, Carol Sze Ki Lin

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33 Citations (Scopus)


Textile waste contains biodegradable fraction which can be used as an alternative feedstock for succinic acid production. The feasibility of bioconversion of biochar-treated textile waste hydrolysate into succinic acid was evaluated. A substrate loading of 9% was applied to hydrolyze mixed textile waste and the resultant glucose-rich hydrolysate with dyestuff was collected after enzymatic hydrolysis. Biochar from different pyrolysis temperatures (400–700 °C)and different dosages of the selected biochar (1–5%)were applied to remove colorants. The results showed that biochar had a good performance at a dosage of 2 w/w % on removal of colorant inhibitors. There was no negative effect observed during the subsequent fermentation. After optimization of fermentation media in shake flasks, the resultant succinic acid titer reached 19.6 g/L with a SA yield of 0.76 g/g, supplemented with 30 g/L tryptone. The in situ fibrous bed bioreactor (isFBB)can further improve SA titer, up to 28.8 g/L corresponding to a yield of 0.61 g/g without pH control. Furthermore, a shorter lag phase during succinic acid fermentation in isFBB was observed. It can be concluded that a novel textile waste-based biorefinery approach for succinic acid production aided by biochar sorption was successfully developed.

Original languageEnglish
Article number107249
JournalBiochemical Engineering Journal
Publication statusPublished - 2019 Sept 15

Bibliographical note

Funding Information:
The authors would like to gratefully acknowledge the Applied Research Grant from City University of Hong Kong (Project No. CityU 9667167). This study was also jointly supported by the National Key Technology Support Program of China (No. 2015BAD15B06 ), and the International Cooperation Project of Shanghai Municipal Science and Technology Commission (No. 18230710700 ). Sincere appreciation is dedicated to Dr Liu Hao from South China University of Technology for providing textile waste pretreatment.

Publisher Copyright:
© 2019 Elsevier B.V.


  • Biochar
  • In situ fibrous bed bioreactor
  • Succinic acid
  • Textile waste
  • Yarrowia lipolytica

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Environmental Engineering
  • Biomedical Engineering


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