Efficient Synthesis of Food-Derived Antioxidant l -Ergothioneine by Engineered Corynebacterium glutamicum

Minhye Kim; Da Woon Jeong; Jun Won Oh; Hyun Jin Jeong; Young Jin Ko; Sung Eun Park; Sung Ok Han

doi:10.1021/acs.jafc.1c07541

Efficient Synthesis of Food-Derived Antioxidant l -Ergothioneine by Engineered Corynebacterium glutamicum

Minhye Kim, Da Woon Jeong, Jun Won Oh, Hyun Jin Jeong, Young Jin Ko, Sung Eun Park, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

l-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and l-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes.

Original language	English
Pages (from-to)	1516-1524
Number of pages	9
Journal	Journal of agricultural and food chemistry
Volume	70
Issue number	5
DOIs	https://doi.org/10.1021/acs.jafc.1c07541
Publication status	Published - 2022 Feb 9

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korean (NRF) grant funded by the Korean government (MSIT) (Nos 2021R1A2C2006944 and 2020M1A2A2080850) and supported by a Korea University Grant.

Publisher Copyright:
© 2022 American Chemical Society.

Keywords

l-cysteine pathway
l-ergothioneine
l-ergothioneine synthetic pathway
pentose phosphate pathway
sulfur assimilation

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

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10.1021/acs.jafc.1c07541

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title = "Efficient Synthesis of Food-Derived Antioxidant l -Ergothioneine by Engineered Corynebacterium glutamicum",

abstract = "l-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and l-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes. ",

keywords = "l-cysteine pathway, l-ergothioneine, l-ergothioneine synthetic pathway, pentose phosphate pathway, sulfur assimilation",

author = "Minhye Kim and Jeong, {Da Woon} and Oh, {Jun Won} and Jeong, {Hyun Jin} and Ko, {Young Jin} and Park, {Sung Eun} and Han, {Sung Ok}",

note = "Funding Information: This work was supported by the National Research Foundation of Korean (NRF) grant funded by the Korean government (MSIT) (Nos 2021R1A2C2006944 and 2020M1A2A2080850) and supported by a Korea University Grant. Publisher Copyright: {\textcopyright} 2022 American Chemical Society.",

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AU - Kim, Minhye

AU - Jeong, Da Woon

AU - Oh, Jun Won

AU - Jeong, Hyun Jin

AU - Ko, Young Jin

AU - Park, Sung Eun

AU - Han, Sung Ok

N1 - Funding Information: This work was supported by the National Research Foundation of Korean (NRF) grant funded by the Korean government (MSIT) (Nos 2021R1A2C2006944 and 2020M1A2A2080850) and supported by a Korea University Grant. Publisher Copyright: © 2022 American Chemical Society.

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Y1 - 2022/2/9

N2 - l-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and l-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes.

AB - l-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and l-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes.

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Efficient Synthesis of Food-Derived Antioxidant l -Ergothioneine by Engineered Corynebacterium glutamicum

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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