TY - JOUR
T1 - Efficient Synthesis of Food-Derived Antioxidant l -Ergothioneine by Engineered Corynebacterium glutamicum
AU - Kim, Minhye
AU - Jeong, Da Woon
AU - Oh, Jun Won
AU - Jeong, Hyun Jin
AU - Ko, Young Jin
AU - Park, Sung Eun
AU - Han, Sung Ok
N1 - Funding Information:
This work was supported by the National Research Foundation of Korean (NRF) grant funded by the Korean government (MSIT) (Nos 2021R1A2C2006944 and 2020M1A2A2080850) and supported by a Korea University Grant.
Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/2/9
Y1 - 2022/2/9
N2 - l-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and l-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes.
AB - l-Ergothioneine (EGT) is a strong antioxidant used in industry, and it is commonly extracted from mushrooms; however, its production is limited. As an alternative, we developed metabolically engineered Corynebacterium glutamicum with reinforced sulfur assimilation and pentose phosphate pathways, which led to the accumulation of 45.0 and 63.2 mg/L EGT, respectively. Additionally, the overexpression of cysEKR resulted in further promoted EGT production in ET4 (66.5 mg/L) and ET7 (85.0 mg/L). Based on this result, we developed the strain ET11, in which all sulfur assimilatory, PP, and l-cysteine synthetic pathways were reinforced, and it synthesized 264.4 mg/L EGT. This study presents the first strategy for EGT synthesis that does not require precursor addition in C. glutamicum, and the production time was shortened. In addition, the synthesized EGT showed high radical scavenging activity (70.7%), thus confirming its antioxidant function. Consequently, this study showed the possibility of EGT commercialization by overcoming the limitations of industrial processes.
KW - l-cysteine pathway
KW - l-ergothioneine
KW - l-ergothioneine synthetic pathway
KW - pentose phosphate pathway
KW - sulfur assimilation
UR - http://www.scopus.com/inward/record.url?scp=85124073990&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.1c07541
DO - 10.1021/acs.jafc.1c07541
M3 - Article
C2 - 35088592
AN - SCOPUS:85124073990
SN - 0021-8561
VL - 70
SP - 1516
EP - 1524
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 5
ER -