Efficient Synthesis of Stearidonic Acid Enriched Triacylglycerol from Ahiflower Seed Oil via a Two-Step Enzyme Reaction

Changhwan Ju, Yu Jin Lee, Hui Su Yoon, Byung Hee Kim, In Hwan Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Stearidonic acid (SDA) is a plant-based n-3 polyunsaturated fatty acid with multiple biological activities. The enrichment of SDA and synthesis of triacylglycerol (TAG) were carried out consecutively via two lipase-catalyzed reactions, hydrolysis, and esterification. First, SDA was enriched into a glyceride fraction from ahiflower seed oil by Candida rugosa lipase-catalyzed hydrolysis. Under the optimum conditions of 35℃, 0.1% lipase powder of Lipase OF, and 50% buffer solution (based on the weight of total substrate), SDA was enriched from 21.6 to 40.7 wt% in glyceride fraction. SDA-enriched TAG was then synthesized from the SDA-enriched glyceride and the SDA-enriched fatty acid via esterification using an in-house immobilized lipase as a biocatalyst. The SDA-enriched fatty acid was obtained from part of the SDA-enriched glyceride by saponification and the in-house immobilized lipase was prepared from Eversa® Transform 2.0 using Lewatit VP OC 1600 as a carrier. The optimum reaction conditions for the synthesis of TAG were a temperature of 50℃, an enzyme loading of 10%, and a vacuum of 10 mmHg. A maximum conversion to TAG of ca. 94% was achieved after 12 h under the optimum conditions.

Original languageEnglish
Pages (from-to)1679-1688
Number of pages10
JournalJournal of Oleo Science
Issue number11
Publication statusPublished - 2022

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(NRF-2021R1A2C1003128).

Publisher Copyright:
© 2022 by Japan Oil Chemists’ Society.


  • Candida rugosa
  • Eversa® transform 2.0
  • ahiflower seed oil
  • stearidonic acid
  • triacylglycerol

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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