Efficient three-dimensional reconstruction of synapse with high-voltage electron microscopy

Joo Lee Kea, Chang Hyun Park, Im Joo Rhyu

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Three-dimensional (3-D) information on nervous tissue is essential for the understanding of brain function. Especially, 3-D synaptic analyses on serial ultrathin sections with transmission electron microscopy (TEM) have contributed to the knowledge on neural plasticity associated with various pathophysiological conditions. The 3-D reconstruction procedures, however, not only require a great amount of expertise but also include time-consuming processes. Here, we carried out computer-assisted 3-D reconstruction of parallel fibre-Purkinje cell synapses based on 250 nm serial sections using high-voltage electron microscopy (HVEM). The 3-D synapse models were constructed more efficiently and rapidly compared with conventional serial TEM reconstruction. This result suggests that 3-D reconstruction with thicker sections and HVEM is a useful method to study synaptic connectivity.

Original languageEnglish
Pages (from-to)139-141
Number of pages3
JournalJournal of Electron Microscopy
Issue number2
Publication statusPublished - 2005 Apr

Bibliographical note

Funding Information:
The authors would like to thank Dr Kiyoshi Hama for helpful comments on the manuscript. We appreciate the strong support of the HVEM group at KBSI, Dae-Jeon, South Korea. This work was supported by Brain Korea 21 Project for Biomedical Science and grant from the Korean Health 21 R&D Project, Korean Ministry of Health and Welfare (HMP-00-GN-01-0002).


  • 3-D reconstruction
  • Cerebellum
  • HVEM
  • Purkinje cell
  • Synapse

ASJC Scopus subject areas

  • Instrumentation


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