Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway

Nami Kim; Mi Sun Kang; Miso Nam; Shin Ae Kim; Geum Sook Hwang; Hyeon Soo Kim

doi:10.3390/ijms20194751

Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway

Nami Kim, Mi Sun Kang, Miso Nam, Shin Ae Kim, Geum Sook Hwang, Hyeon Soo Kim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

EPA, an omega-3 polyunsaturated fatty acid, exerts beneficial effects on human health. However, the molecular mechanisms underlying EPA function are poorly understood. The object was to illuminate molecular mechanism underlying EPA’s role. Here, 1H-NMR-based metabolic analysis showed enhanced branched-chain amino acids (BCAAs) and lactate following EPA treatment in skeletal muscle cells. EPA regulated mitochondrial oxygen consumption rate. Furthermore, EPA induced calcium/calmodulin-dependent protein kinase kinase (CaMKK) through the generation of intracellular calcium. This induced the phosphorylation of AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (p38 MAPK) that led to glucose uptake, and the translocation of glucose transporter type 4 (GLUT4) in muscles. In conclusion, EPA exerts benign effects on glucose through the activation of AMPK-p38 MAPK signaling pathways in skeletal muscles.

Original language	English
Article number	4751
Journal	International journal of molecular sciences
Volume	20
Issue number	19
DOIs	https://doi.org/10.3390/ijms20194751
Publication status	Published - 2019 Oct 1

Keywords

AMPK
EPA
GLUT4
Oxygen consumption

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/ijms20194751

Cite this

@article{96bbb1836490485a9ee571a5d6c34b08,

title = "Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway",

abstract = "EPA, an omega-3 polyunsaturated fatty acid, exerts beneficial effects on human health. However, the molecular mechanisms underlying EPA function are poorly understood. The object was to illuminate molecular mechanism underlying EPA{\textquoteright}s role. Here, 1H-NMR-based metabolic analysis showed enhanced branched-chain amino acids (BCAAs) and lactate following EPA treatment in skeletal muscle cells. EPA regulated mitochondrial oxygen consumption rate. Furthermore, EPA induced calcium/calmodulin-dependent protein kinase kinase (CaMKK) through the generation of intracellular calcium. This induced the phosphorylation of AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (p38 MAPK) that led to glucose uptake, and the translocation of glucose transporter type 4 (GLUT4) in muscles. In conclusion, EPA exerts benign effects on glucose through the activation of AMPK-p38 MAPK signaling pathways in skeletal muscles.",

keywords = "AMPK, EPA, GLUT4, Oxygen consumption",

author = "Nami Kim and Kang, {Mi Sun} and Miso Nam and Kim, {Shin Ae} and Hwang, {Geum Sook} and Kim, {Hyeon Soo}",

note = "Funding Information: This study was supported by the National Research Foundation of Korea, which is funded by the Korean government (NRF-2018R1E1A2A02085743). This study was also supported by the National Research Foundation funded by the Ministry of Science, ICT, & Future Planning, Korea (2013M3A9B6046418), the National Council of Science and Technology (CAP-12-2-KBSI), and the Korea Basic Science Institute (C37705). Funding Information: Funding: This study was supported by the National Research Foundation of Korea, which is funded by the Korean government (NRF-2018R1E1A2A02085743). This study was also supported by the National Research Foundation funded by the Ministry of Science, ICT, & Future Planning, Korea (2013M3A9B6046418), the National Council of Science and Technology (CAP-12–2-KBSI), and the Korea Basic Science Institute (C37705). Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

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doi = "10.3390/ijms20194751",

language = "English",

volume = "20",

journal = "International Journal of Molecular Sciences",

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T1 - Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway

AU - Kim, Nami

AU - Kang, Mi Sun

AU - Nam, Miso

AU - Kim, Shin Ae

AU - Hwang, Geum Sook

AU - Kim, Hyeon Soo

N1 - Funding Information: This study was supported by the National Research Foundation of Korea, which is funded by the Korean government (NRF-2018R1E1A2A02085743). This study was also supported by the National Research Foundation funded by the Ministry of Science, ICT, & Future Planning, Korea (2013M3A9B6046418), the National Council of Science and Technology (CAP-12-2-KBSI), and the Korea Basic Science Institute (C37705). Funding Information: Funding: This study was supported by the National Research Foundation of Korea, which is funded by the Korean government (NRF-2018R1E1A2A02085743). This study was also supported by the National Research Foundation funded by the Ministry of Science, ICT, & Future Planning, Korea (2013M3A9B6046418), the National Council of Science and Technology (CAP-12–2-KBSI), and the Korea Basic Science Institute (C37705). Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - EPA, an omega-3 polyunsaturated fatty acid, exerts beneficial effects on human health. However, the molecular mechanisms underlying EPA function are poorly understood. The object was to illuminate molecular mechanism underlying EPA’s role. Here, 1H-NMR-based metabolic analysis showed enhanced branched-chain amino acids (BCAAs) and lactate following EPA treatment in skeletal muscle cells. EPA regulated mitochondrial oxygen consumption rate. Furthermore, EPA induced calcium/calmodulin-dependent protein kinase kinase (CaMKK) through the generation of intracellular calcium. This induced the phosphorylation of AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (p38 MAPK) that led to glucose uptake, and the translocation of glucose transporter type 4 (GLUT4) in muscles. In conclusion, EPA exerts benign effects on glucose through the activation of AMPK-p38 MAPK signaling pathways in skeletal muscles.

AB - EPA, an omega-3 polyunsaturated fatty acid, exerts beneficial effects on human health. However, the molecular mechanisms underlying EPA function are poorly understood. The object was to illuminate molecular mechanism underlying EPA’s role. Here, 1H-NMR-based metabolic analysis showed enhanced branched-chain amino acids (BCAAs) and lactate following EPA treatment in skeletal muscle cells. EPA regulated mitochondrial oxygen consumption rate. Furthermore, EPA induced calcium/calmodulin-dependent protein kinase kinase (CaMKK) through the generation of intracellular calcium. This induced the phosphorylation of AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (p38 MAPK) that led to glucose uptake, and the translocation of glucose transporter type 4 (GLUT4) in muscles. In conclusion, EPA exerts benign effects on glucose through the activation of AMPK-p38 MAPK signaling pathways in skeletal muscles.

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ER -

Eicosapentaenoic Acid (EPA) modulates glucose metabolism by targeting AMP-Activated Protein Kinase (AMPK) pathway

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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