Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction

Thao Thi Hien Pham; Sang Jun Sim

doi:10.1007/s11051-009-9600-7

Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction

Thao Thi Hien Pham, Sang Jun Sim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

An electrochemical impedance immunosensor was developed for detecting the immunological interaction between human immunoglobulin (IgG) and protein A from Staphylococcus aureus based on the immobilization of human IgG on the surface of modified gold-coated magnetic nanoparticles. The nanoparticles with an Au shell and Fe oxide cores were functionalized by a self-assembled monolayer of 11-mercaptoundecanoic acid. The electrochemical analysis was conducted on the modified magnetic carbon paste electrodes with the nanoparticles. The magnetic nanoparticles were attached to the surface of the magnetic carbon paste electrodes via magnetic force. The cyclic voltammetry technique and electrochemical impedance spectroscopy measurements of the magnetic carbon paste electrodes coated with magnetic nanoparticles-human IgG complex showed changes in its alternating current (AC) response both after the modification of the surface of the electrode and the addition of protein A. The immunological interaction between human IgG on the surface of the modified magnetic carbon paste electrodes and protein A in the solution could be successfully monitored.

Original language	English
Pages (from-to)	227-235
Number of pages	9
Journal	Journal of Nanoparticle Research
Volume	12
Issue number	1
DOIs	https://doi.org/10.1007/s11051-009-9600-7
Publication status	Published - 2010 Jan
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) of the Republic of Korea.

Keywords

Cyclic voltammetry
Electrochemical impedance spectroscopy
Gold-coated magnetic nanoparticles
Immunological interaction
Nanomedicine

ASJC Scopus subject areas

Bioengineering
Atomic and Molecular Physics, and Optics
General Chemistry
Modelling and Simulation
General Materials Science
Condensed Matter Physics

Access to Document

10.1007/s11051-009-9600-7

Cite this

@article{2f4b526b6802483298c5a59ad29399cd,

title = "Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction",

abstract = "An electrochemical impedance immunosensor was developed for detecting the immunological interaction between human immunoglobulin (IgG) and protein A from Staphylococcus aureus based on the immobilization of human IgG on the surface of modified gold-coated magnetic nanoparticles. The nanoparticles with an Au shell and Fe oxide cores were functionalized by a self-assembled monolayer of 11-mercaptoundecanoic acid. The electrochemical analysis was conducted on the modified magnetic carbon paste electrodes with the nanoparticles. The magnetic nanoparticles were attached to the surface of the magnetic carbon paste electrodes via magnetic force. The cyclic voltammetry technique and electrochemical impedance spectroscopy measurements of the magnetic carbon paste electrodes coated with magnetic nanoparticles-human IgG complex showed changes in its alternating current (AC) response both after the modification of the surface of the electrode and the addition of protein A. The immunological interaction between human IgG on the surface of the modified magnetic carbon paste electrodes and protein A in the solution could be successfully monitored.",

keywords = "Cyclic voltammetry, Electrochemical impedance spectroscopy, Gold-coated magnetic nanoparticles, Immunological interaction, Nanomedicine",

author = "Pham, {Thao Thi Hien} and Sim, {Sang Jun}",

note = "Funding Information: Acknowledgments This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) of the Republic of Korea.",

year = "2010",

month = jan,

doi = "10.1007/s11051-009-9600-7",

language = "English",

volume = "12",

pages = "227--235",

journal = "Journal of Nanoparticle Research",

issn = "1388-0764",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction

AU - Pham, Thao Thi Hien

AU - Sim, Sang Jun

N1 - Funding Information: Acknowledgments This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program grant funded by the Korea government (MEST) (grant no. R0A-2008-000-20078-0) of the Republic of Korea.

PY - 2010/1

Y1 - 2010/1

N2 - An electrochemical impedance immunosensor was developed for detecting the immunological interaction between human immunoglobulin (IgG) and protein A from Staphylococcus aureus based on the immobilization of human IgG on the surface of modified gold-coated magnetic nanoparticles. The nanoparticles with an Au shell and Fe oxide cores were functionalized by a self-assembled monolayer of 11-mercaptoundecanoic acid. The electrochemical analysis was conducted on the modified magnetic carbon paste electrodes with the nanoparticles. The magnetic nanoparticles were attached to the surface of the magnetic carbon paste electrodes via magnetic force. The cyclic voltammetry technique and electrochemical impedance spectroscopy measurements of the magnetic carbon paste electrodes coated with magnetic nanoparticles-human IgG complex showed changes in its alternating current (AC) response both after the modification of the surface of the electrode and the addition of protein A. The immunological interaction between human IgG on the surface of the modified magnetic carbon paste electrodes and protein A in the solution could be successfully monitored.

AB - An electrochemical impedance immunosensor was developed for detecting the immunological interaction between human immunoglobulin (IgG) and protein A from Staphylococcus aureus based on the immobilization of human IgG on the surface of modified gold-coated magnetic nanoparticles. The nanoparticles with an Au shell and Fe oxide cores were functionalized by a self-assembled monolayer of 11-mercaptoundecanoic acid. The electrochemical analysis was conducted on the modified magnetic carbon paste electrodes with the nanoparticles. The magnetic nanoparticles were attached to the surface of the magnetic carbon paste electrodes via magnetic force. The cyclic voltammetry technique and electrochemical impedance spectroscopy measurements of the magnetic carbon paste electrodes coated with magnetic nanoparticles-human IgG complex showed changes in its alternating current (AC) response both after the modification of the surface of the electrode and the addition of protein A. The immunological interaction between human IgG on the surface of the modified magnetic carbon paste electrodes and protein A in the solution could be successfully monitored.

KW - Cyclic voltammetry

KW - Electrochemical impedance spectroscopy

KW - Gold-coated magnetic nanoparticles

KW - Immunological interaction

KW - Nanomedicine

UR - http://www.scopus.com/inward/record.url?scp=75049085169&partnerID=8YFLogxK

U2 - 10.1007/s11051-009-9600-7

DO - 10.1007/s11051-009-9600-7

M3 - Article

AN - SCOPUS:75049085169

SN - 1388-0764

VL - 12

SP - 227

EP - 235

JO - Journal of Nanoparticle Research

JF - Journal of Nanoparticle Research

IS - 1

ER -

Electrochemical analysis of gold-coated magnetic nanoparticles for detecting immunological interaction

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this