Abstract
We developed an electrochemical DNA sensor based on gold electrode arrays modified with a thiol linker synthesized by novel solid-phase strategy, and could effectively detect the aging-dependant T414G mutation of mitochondrial DNA. To electrochemically detect the single nucleotide polymorphism (SNP), the gold electrode arrays were patterned on glass wafers, and the thiol linker facilely synthesized on the solid support was assembled on the gold electrode surface. On the SAM electrode surface, three kinds of DNA probes, T414G-T probe, T414G-G probe and control R probe, were covalently immobilized. Using the cyclic voltametry (CV), we could not only discriminate between full-matched DNA and single-mismatched DNA successfully, but also confirm the effectiveness of the synthesized thiol linkers for reducing the nonspecific DNA binding.
Original language | English |
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Pages (from-to) | 266-274 |
Number of pages | 9 |
Journal | Biochip Journal |
Volume | 3 |
Issue number | 3 |
Publication status | Published - 2009 |
Keywords
- Electrochemical DNA sensor
- Gold electrode arrays
- Mitochondrial DNA
- Single nucleotide polymorphism
- Solid-phase synthesis
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Electrical and Electronic Engineering