Electrochemical properties of enzyme electrode covalently immobilized on a graphite oxide/cobalt hydroxide/chitosan composite mediator for biofuel cells

Ji Hyun Yang; Hyeong Ryeol Kim; Ja Hyun Lee; Joon Hyung Jin; Hee Uk Lee; Seung Wook Kim

doi:10.1016/j.ijhydene.2020.03.084

Electrochemical properties of enzyme electrode covalently immobilized on a graphite oxide/cobalt hydroxide/chitosan composite mediator for biofuel cells

Ji Hyun Yang, Hyeong Ryeol Kim, Ja Hyun Lee, Joon Hyung Jin, Hee Uk Lee, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A critical factor for the performance of a biofuel cell is an immobilization of the redox enzyme for continuous catalytic reaction and efficient electron transfer. However, the main obstacle associated with enzyme electrode is the reduced surface area for the accommodation of enzymes, leading to poor power output. This study aimed to optimize the efficient electrical communication for glucose oxidase (GOx) on the surface of a graphite oxide/cobalt hydroxide/chitosan composite as mediator, thereby enhancing the generation of power output. Immobilization efficiency was affected by the different concentrations of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Also, the surface of enzyme electrode was observed by XPS, Raman, and AFM, respectively. The electrochemical characterization showed that the immobilized GOx possesses the highest activity at EDC:NHS(40:80 mM) concentration. The power output under the optimal condition was found to be 2.24 mWcm⁻² of power density using the three-electrode cell in 0.1 M PBS solution at room temperature.

Original language	English
Pages (from-to)	3251-3258
Number of pages	8
Journal	International Journal of Hydrogen Energy
Volume	46
Issue number	4
DOIs	https://doi.org/10.1016/j.ijhydene.2020.03.084
Publication status	Published - 2021 Jan 14

Bibliographical note

Funding Information:
This work was supported by the BK21 Plus grant funded by Korea University and a grant ( NRF-2019R1A2C1006793 , NRF-2018R1D1A1B07048927 and NRF-2014R1A2A2A01007321 ) of the National Research Foundation of Korea (NRF) funded by the Korean government.

Publisher Copyright:
© 2020 Hydrogen Energy Publications LLC

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Keywords

Biofuel cell
Electron transfer
Enzyme immobilization
Glucose oxidase
Mediator

ASJC Scopus subject areas

Renewable Energy, Sustainability and the Environment
Fuel Technology
Condensed Matter Physics
Energy Engineering and Power Technology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijhydene.2020.03.084

Cite this

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title = "Electrochemical properties of enzyme electrode covalently immobilized on a graphite oxide/cobalt hydroxide/chitosan composite mediator for biofuel cells",

abstract = "A critical factor for the performance of a biofuel cell is an immobilization of the redox enzyme for continuous catalytic reaction and efficient electron transfer. However, the main obstacle associated with enzyme electrode is the reduced surface area for the accommodation of enzymes, leading to poor power output. This study aimed to optimize the efficient electrical communication for glucose oxidase (GOx) on the surface of a graphite oxide/cobalt hydroxide/chitosan composite as mediator, thereby enhancing the generation of power output. Immobilization efficiency was affected by the different concentrations of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Also, the surface of enzyme electrode was observed by XPS, Raman, and AFM, respectively. The electrochemical characterization showed that the immobilized GOx possesses the highest activity at EDC:NHS(40:80 mM) concentration. The power output under the optimal condition was found to be 2.24 mWcm−2 of power density using the three-electrode cell in 0.1 M PBS solution at room temperature.",

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note = "Funding Information: This work was supported by the BK21 Plus grant funded by Korea University and a grant ( NRF-2019R1A2C1006793 , NRF-2018R1D1A1B07048927 and NRF-2014R1A2A2A01007321 ) of the National Research Foundation of Korea (NRF) funded by the Korean government. Publisher Copyright: {\textcopyright} 2020 Hydrogen Energy Publications LLC Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

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AU - Yang, Ji Hyun

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AU - Lee, Ja Hyun

AU - Jin, Joon Hyung

AU - Lee, Hee Uk

AU - Kim, Seung Wook

N1 - Funding Information: This work was supported by the BK21 Plus grant funded by Korea University and a grant ( NRF-2019R1A2C1006793 , NRF-2018R1D1A1B07048927 and NRF-2014R1A2A2A01007321 ) of the National Research Foundation of Korea (NRF) funded by the Korean government. Publisher Copyright: © 2020 Hydrogen Energy Publications LLC Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/1/14

Y1 - 2021/1/14

N2 - A critical factor for the performance of a biofuel cell is an immobilization of the redox enzyme for continuous catalytic reaction and efficient electron transfer. However, the main obstacle associated with enzyme electrode is the reduced surface area for the accommodation of enzymes, leading to poor power output. This study aimed to optimize the efficient electrical communication for glucose oxidase (GOx) on the surface of a graphite oxide/cobalt hydroxide/chitosan composite as mediator, thereby enhancing the generation of power output. Immobilization efficiency was affected by the different concentrations of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Also, the surface of enzyme electrode was observed by XPS, Raman, and AFM, respectively. The electrochemical characterization showed that the immobilized GOx possesses the highest activity at EDC:NHS(40:80 mM) concentration. The power output under the optimal condition was found to be 2.24 mWcm−2 of power density using the three-electrode cell in 0.1 M PBS solution at room temperature.

AB - A critical factor for the performance of a biofuel cell is an immobilization of the redox enzyme for continuous catalytic reaction and efficient electron transfer. However, the main obstacle associated with enzyme electrode is the reduced surface area for the accommodation of enzymes, leading to poor power output. This study aimed to optimize the efficient electrical communication for glucose oxidase (GOx) on the surface of a graphite oxide/cobalt hydroxide/chitosan composite as mediator, thereby enhancing the generation of power output. Immobilization efficiency was affected by the different concentrations of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Also, the surface of enzyme electrode was observed by XPS, Raman, and AFM, respectively. The electrochemical characterization showed that the immobilized GOx possesses the highest activity at EDC:NHS(40:80 mM) concentration. The power output under the optimal condition was found to be 2.24 mWcm−2 of power density using the three-electrode cell in 0.1 M PBS solution at room temperature.

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KW - Electron transfer

KW - Enzyme immobilization

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KW - Mediator

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Electrochemical properties of enzyme electrode covalently immobilized on a graphite oxide/cobalt hydroxide/chitosan composite mediator for biofuel cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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