TY - JOUR
T1 - Electrokinetic size-based spatial separation of micro/nanospheres using paper-based 3d origami preconcentrator
AU - Han, Sung Il
AU - Lee, Dohwan
AU - Kim, Hyerin
AU - Yoo, Yong Kyoung
AU - Kim, Cheonjung
AU - Lee, Junwoo
AU - Kim, Kang Hyeon
AU - Kim, Hyungsuk
AU - Lee, Dongho
AU - Hwang, Kyo Seon
AU - Yoon, Dae Sung
AU - Lee, Jeong Hoon
N1 - Funding Information:
We are very grateful for the financial support received from the National Research Foundation of Korea, Grant (NRF-2018R1D1A1A09084044). We also gratefully acknowledge the Basic Science Research Program, through the National Research Foundation of Korea (NRF, Grant no. 2019R1A2B5B01070617, and NRF, Grant no. 2018M3C1B7020722). J.H.L. was partially supported by a research grant from Kwangwoon University in 2019.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/8/20
Y1 - 2019/8/20
N2 - Sample preparation steps (e.g., preconcentration and separation) are key to enhancing sensitivity and reliability in biomedical and analytical chemistry. However, conventional methods (e.g., ultracentrifugation) cause significant loss of sample as well as their contamination. In this study, we developed a paper-based three-dimensional (3D) origami ion concentration polarization preconcentrator (POP) for highly efficient and facile sample preparation. The unique design of POP enables simultaneous preconcentration and spatial separation of target analytes rapidly and economically. The POP comprises accordion-like multifolded layers with convergent wicking areas that can separate analytes based on their sizes in different layers, which can then be easily isolated by unfolding the POP. We first demonstrated 100-fold preconcentration of albumin and its isolation on the specific layers. Then, we demonstrated the simultaneous preconcentration and spatial separation of microspheres of three different sizes (with diameters of 0.02, 0.2, and 2 μm) on the different layers.
AB - Sample preparation steps (e.g., preconcentration and separation) are key to enhancing sensitivity and reliability in biomedical and analytical chemistry. However, conventional methods (e.g., ultracentrifugation) cause significant loss of sample as well as their contamination. In this study, we developed a paper-based three-dimensional (3D) origami ion concentration polarization preconcentrator (POP) for highly efficient and facile sample preparation. The unique design of POP enables simultaneous preconcentration and spatial separation of target analytes rapidly and economically. The POP comprises accordion-like multifolded layers with convergent wicking areas that can separate analytes based on their sizes in different layers, which can then be easily isolated by unfolding the POP. We first demonstrated 100-fold preconcentration of albumin and its isolation on the specific layers. Then, we demonstrated the simultaneous preconcentration and spatial separation of microspheres of three different sizes (with diameters of 0.02, 0.2, and 2 μm) on the different layers.
UR - http://www.scopus.com/inward/record.url?scp=85070865753&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.9b02201
DO - 10.1021/acs.analchem.9b02201
M3 - Article
C2 - 31340120
AN - SCOPUS:85070865753
SN - 0003-2700
VL - 91
SP - 10744
EP - 10749
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 16
ER -