Electrokinetic size-based spatial separation of micro/nanospheres using paper-based 3d origami preconcentrator

Sung Il Han, Dohwan Lee, Hyerin Kim, Yong Kyoung Yoo, Cheonjung Kim, Junwoo Lee, Kang Hyeon Kim, Hyungsuk Kim, Dongho Lee, Kyo Seon Hwang, Dae Sung Yoon, Jeong Hoon Lee

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Sample preparation steps (e.g., preconcentration and separation) are key to enhancing sensitivity and reliability in biomedical and analytical chemistry. However, conventional methods (e.g., ultracentrifugation) cause significant loss of sample as well as their contamination. In this study, we developed a paper-based three-dimensional (3D) origami ion concentration polarization preconcentrator (POP) for highly efficient and facile sample preparation. The unique design of POP enables simultaneous preconcentration and spatial separation of target analytes rapidly and economically. The POP comprises accordion-like multifolded layers with convergent wicking areas that can separate analytes based on their sizes in different layers, which can then be easily isolated by unfolding the POP. We first demonstrated 100-fold preconcentration of albumin and its isolation on the specific layers. Then, we demonstrated the simultaneous preconcentration and spatial separation of microspheres of three different sizes (with diameters of 0.02, 0.2, and 2 μm) on the different layers.

Original languageEnglish
Pages (from-to)10744-10749
Number of pages6
JournalAnalytical chemistry
Volume91
Issue number16
DOIs
Publication statusPublished - 2019 Aug 20

Bibliographical note

Funding Information:
We are very grateful for the financial support received from the National Research Foundation of Korea, Grant (NRF-2018R1D1A1A09084044). We also gratefully acknowledge the Basic Science Research Program, through the National Research Foundation of Korea (NRF, Grant no. 2019R1A2B5B01070617, and NRF, Grant no. 2018M3C1B7020722). J.H.L. was partially supported by a research grant from Kwangwoon University in 2019.

Publisher Copyright:
© 2019 American Chemical Society.

ASJC Scopus subject areas

  • Analytical Chemistry

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