Electroosmotic Effects on Sample Concentration at the Interface of a Micro/Nanochannel

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Electroosmotic effect on electropreconcentration of analytes was investigated at the micro/nanochannel interface for a series of 1-D glass nanochannels with depths of 72, 54, 29, and 9 nm. The electric double layer approaches overlap conditions as the nanochannel depth decreases, suppressing the electroosmotic flow. The nanochannels' electroosmotic flows (μeonano) were determined and compared to the analyte's (fluorescein) electrophoretic mobility (μep). For the instances where μeonano > μep, the analytes were concentrated on the anodic side of the nanochannel (72, 54, and 29 nm deep channels), whereas when μeonano < μep, the analyte was concentrated on the cathodic side of the nanochannel (9 nm deep channel). In order to maintain a stable concentrated sample plug, a "drain" channel was incorporated for increasing the nanochannel electroosmotic flow while decreasing the sample channel electroosmotic flow. A sample preconcentration rate of over 20-fold per second was achieved. Finally, fundamental limits of the nanochannel-based preconcentration are discussed and experimentally demonstrated.

Original languageEnglish
Pages (from-to)8924-8930
Number of pages7
JournalAnalytical chemistry
Issue number17
Publication statusPublished - 2017 Sept 5

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) (NRF-2013M3A6A4046061, NRF-2012-0009556, and NRF-2011-0031866).

ASJC Scopus subject areas

  • Analytical Chemistry


Dive into the research topics of 'Electroosmotic Effects on Sample Concentration at the Interface of a Micro/Nanochannel'. Together they form a unique fingerprint.

Cite this