Electrophysiological evidence for functional astrocytic P2X3 receptors in the mouse trigeminal caudal nucleus

Jaekwang Lee; Jin Young Bae; Justin J. Lee; Yong Chul Bae

doi:10.5607/en.2018.27.2.88

Electrophysiological evidence for functional astrocytic P2X₃ receptors in the mouse trigeminal caudal nucleus

Jaekwang Lee, Jin Young Bae, Justin J. Lee, Yong Chul Bae

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Recently, we reported that astrocytes in the trigeminal caudal nucleus (Vc) of the brain stem express a purinergic receptor P2X₃, which is involved in the craniofacial pathologic pain. Although we observed protein expression of P2X₃ receptors (P2X₃ Rs) in the astrocyte of the Vc, it is still unclear that astrocyte has functional P2X₃Rs in Vc. To address this issue, we recorded asrtocytic P2X₃Rs by using whole cell voltage-clamp recording in the Vc of the GFAP-GFP mice, which was used as a guide to astrocytes with green fluorescence. While measuring voltage ramp-induced astrocytic membrane current, we found the amplitude of the current was increased when we applied P2-purinoreceptor agonist, a,ß-meATP. This increase was blocked by co-application of A317491, P2X₃R antagonist. These results demonstrate that astrocytes in the Vc express functional P2X₃Rs, which might be critical in craniofacial pathologic pain.

Original language	English
Pages (from-to)	88-93
Number of pages	6
Journal	Experimental Neurobiology
Volume	27
Issue number	2
DOIs	https://doi.org/10.5607/en.2018.27.2.88
Publication status	Published - 2018 Apr 1

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2017R1A2B2003561, NRF-2017R1A5A2015391) and by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI17C0952).

Publisher Copyright:
© Experimental Neurobiology 2018.

Keywords

Astrocytes
Electrophysiology
Pain
Purinergic P2X
Trigeminal caudal nucleus

ASJC Scopus subject areas

Clinical Neurology
Cellular and Molecular Neuroscience

Access to Document

10.5607/en.2018.27.2.88

Cite this

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title = "Electrophysiological evidence for functional astrocytic P2X3 receptors in the mouse trigeminal caudal nucleus",

abstract = "Recently, we reported that astrocytes in the trigeminal caudal nucleus (Vc) of the brain stem express a purinergic receptor P2X3, which is involved in the craniofacial pathologic pain. Although we observed protein expression of P2X3 receptors (P2X3 Rs) in the astrocyte of the Vc, it is still unclear that astrocyte has functional P2X3Rs in Vc. To address this issue, we recorded asrtocytic P2X3Rs by using whole cell voltage-clamp recording in the Vc of the GFAP-GFP mice, which was used as a guide to astrocytes with green fluorescence. While measuring voltage ramp-induced astrocytic membrane current, we found the amplitude of the current was increased when we applied P2-purinoreceptor agonist, a,{\ss}-meATP. This increase was blocked by co-application of A317491, P2X3R antagonist. These results demonstrate that astrocytes in the Vc express functional P2X3Rs, which might be critical in craniofacial pathologic pain.",

keywords = "Astrocytes, Electrophysiology, Pain, Purinergic P2X, Trigeminal caudal nucleus",

author = "Jaekwang Lee and Bae, {Jin Young} and Lee, {Justin J.} and Bae, {Yong Chul}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (NRF-2017R1A2B2003561, NRF-2017R1A5A2015391) and by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (HI17C0952). Publisher Copyright: {\textcopyright} Experimental Neurobiology 2018.",

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N2 - Recently, we reported that astrocytes in the trigeminal caudal nucleus (Vc) of the brain stem express a purinergic receptor P2X3, which is involved in the craniofacial pathologic pain. Although we observed protein expression of P2X3 receptors (P2X3 Rs) in the astrocyte of the Vc, it is still unclear that astrocyte has functional P2X3Rs in Vc. To address this issue, we recorded asrtocytic P2X3Rs by using whole cell voltage-clamp recording in the Vc of the GFAP-GFP mice, which was used as a guide to astrocytes with green fluorescence. While measuring voltage ramp-induced astrocytic membrane current, we found the amplitude of the current was increased when we applied P2-purinoreceptor agonist, a,ß-meATP. This increase was blocked by co-application of A317491, P2X3R antagonist. These results demonstrate that astrocytes in the Vc express functional P2X3Rs, which might be critical in craniofacial pathologic pain.

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