Electrostatic interaction effect for human DNA separation with functionalized mesoporous silicas

Hong Kyung Choi; Jeong Ho Chang; Il Hwan Ko; Jin Hyung Lee; Bong Yong Jeong; Jong Hee Kim; Jung Bae Kim

doi:10.1016/j.jssc.2011.02.005

Electrostatic interaction effect for human DNA separation with functionalized mesoporous silicas

Hong Kyung Choi, Jeong Ho Chang, Il Hwan Ko, Jin Hyung Lee, Bong Yong Jeong, Jong Hee Kim, Jung Bae Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

This work describes the development of highly efficient human DNA separation with functionalized mesoporous silica (FMS) materials. To demonstrate the electrostatic interaction effect between the target DNA molecules and FMS, three aminofunctionality types comprised of a mono-, a di-, and a tri-amine functional group were introduced on the inner surfaces of mesoporous silica particles. Systematic characterization of the synthesized materials was achieved by solid-state ²⁹Si and ¹³C-NMR techniques, BET, FT-IR, and XPS. The DNA separation efficiency was explored via the function of the amino-group number, the amount used, and the added NaCl concentration. The DNA adsorption yields were high in terms of the use of triaminofunctionalized FMS at the 10 ng/L level, and the DNA desorption efficiency showed the optimum level at over 3.0 M NaCl concentration. The use of FMS in a DNA separation process provides numerous advantages over the conventional silica-based process.

Original language	English
Pages (from-to)	805-810
Number of pages	6
Journal	Journal of Solid State Chemistry
Volume	184
Issue number	4
DOIs	https://doi.org/10.1016/j.jssc.2011.02.005
Publication status	Published - 2011 Apr

Bibliographical note

Funding Information:
This work was supported by a grant from the Fundmental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea, and partially supported by Korea Institute of Ceramic Engineering and Technology (KICET).

Keywords

Confocal microscope
DNA separation
Electrostatic interaction
Functionalized mesoporous silicas
Solid-state NMR

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Ceramics and Composites
Condensed Matter Physics
Physical and Theoretical Chemistry
Inorganic Chemistry
Materials Chemistry

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10.1016/j.jssc.2011.02.005

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title = "Electrostatic interaction effect for human DNA separation with functionalized mesoporous silicas",

abstract = "This work describes the development of highly efficient human DNA separation with functionalized mesoporous silica (FMS) materials. To demonstrate the electrostatic interaction effect between the target DNA molecules and FMS, three aminofunctionality types comprised of a mono-, a di-, and a tri-amine functional group were introduced on the inner surfaces of mesoporous silica particles. Systematic characterization of the synthesized materials was achieved by solid-state 29Si and 13C-NMR techniques, BET, FT-IR, and XPS. The DNA separation efficiency was explored via the function of the amino-group number, the amount used, and the added NaCl concentration. The DNA adsorption yields were high in terms of the use of triaminofunctionalized FMS at the 10 ng/L level, and the DNA desorption efficiency showed the optimum level at over 3.0 M NaCl concentration. The use of FMS in a DNA separation process provides numerous advantages over the conventional silica-based process.",

keywords = "Confocal microscope, DNA separation, Electrostatic interaction, Functionalized mesoporous silicas, Solid-state NMR",

author = "Choi, {Hong Kyung} and Chang, {Jeong Ho} and Ko, {Il Hwan} and Lee, {Jin Hyung} and Jeong, {Bong Yong} and Kim, {Jong Hee} and Kim, {Jung Bae}",

note = "Funding Information: This work was supported by a grant from the Fundmental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea, and partially supported by Korea Institute of Ceramic Engineering and Technology (KICET).",

year = "2011",

month = apr,

doi = "10.1016/j.jssc.2011.02.005",

language = "English",

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AU - Choi, Hong Kyung

AU - Chang, Jeong Ho

AU - Ko, Il Hwan

AU - Lee, Jin Hyung

AU - Jeong, Bong Yong

AU - Kim, Jong Hee

AU - Kim, Jung Bae

N1 - Funding Information: This work was supported by a grant from the Fundmental R&D Program for Core Technology of Materials funded by the Ministry of Knowledge Economy, Republic of Korea, and partially supported by Korea Institute of Ceramic Engineering and Technology (KICET).

PY - 2011/4

Y1 - 2011/4

N2 - This work describes the development of highly efficient human DNA separation with functionalized mesoporous silica (FMS) materials. To demonstrate the electrostatic interaction effect between the target DNA molecules and FMS, three aminofunctionality types comprised of a mono-, a di-, and a tri-amine functional group were introduced on the inner surfaces of mesoporous silica particles. Systematic characterization of the synthesized materials was achieved by solid-state 29Si and 13C-NMR techniques, BET, FT-IR, and XPS. The DNA separation efficiency was explored via the function of the amino-group number, the amount used, and the added NaCl concentration. The DNA adsorption yields were high in terms of the use of triaminofunctionalized FMS at the 10 ng/L level, and the DNA desorption efficiency showed the optimum level at over 3.0 M NaCl concentration. The use of FMS in a DNA separation process provides numerous advantages over the conventional silica-based process.

AB - This work describes the development of highly efficient human DNA separation with functionalized mesoporous silica (FMS) materials. To demonstrate the electrostatic interaction effect between the target DNA molecules and FMS, three aminofunctionality types comprised of a mono-, a di-, and a tri-amine functional group were introduced on the inner surfaces of mesoporous silica particles. Systematic characterization of the synthesized materials was achieved by solid-state 29Si and 13C-NMR techniques, BET, FT-IR, and XPS. The DNA separation efficiency was explored via the function of the amino-group number, the amount used, and the added NaCl concentration. The DNA adsorption yields were high in terms of the use of triaminofunctionalized FMS at the 10 ng/L level, and the DNA desorption efficiency showed the optimum level at over 3.0 M NaCl concentration. The use of FMS in a DNA separation process provides numerous advantages over the conventional silica-based process.

KW - Confocal microscope

KW - DNA separation

KW - Electrostatic interaction

KW - Functionalized mesoporous silicas

KW - Solid-state NMR

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JO - Journal of Solid State Chemistry

JF - Journal of Solid State Chemistry

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ER -

Electrostatic interaction effect for human DNA separation with functionalized mesoporous silicas

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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