Engineered M13 bacteriophage-enhanced colorimetric detection of allergenic fungi

Sang Min Lee, Seohui Lee, Sang Hyun Lee, Geum Ran Ahn, Byung Yang Lee, Seong Hwan Kim, Minkyung Song, Woo Jae Chung

Research output: Contribution to journalArticlepeer-review


Fungal spores are an important component of bio-aerosols and ubiquitous in indoor environments, leading to adverse health problems. Because concerns about indoor air quality have increased, rapid and sensitive detection methods are needed for personal and public health. In this study, we developed a sensitive colorimetric biosensor for detecting allergenic fungal spores using engineered M13 bacteriophages. Aspergillus niger spore-binding phages, showing high binding affinity to A. niger spores, were identified by phage display screening. The phages were biotinylated on the major coat protein pVIII for binding with streptavidin–HRP conjugates, resulting in enhanced color change. This engineered phage exhibited higher sensitivity and specificity for A. niger spores than conventionally used antibody-based detection systems.

Original languageEnglish
Article number134244
JournalSensors and Actuators, B: Chemical
Publication statusPublished - 2023 Oct 15

Bibliographical note

Funding Information:
This research was supported by Korea Ministry of Education ( 2017R1A6A1A03015642 , 2022R1F1A106325211 ). This work was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute ( KHIDI ), funded by Korea Ministry of Health & Welfare ( HI22C060700 ). This project was also supported by the National Research Foundation ( NRF ) funded by the Korea Ministry of Science and ICT ( 2021R1A2C2014088 ).

Publisher Copyright:
© 2023 Elsevier B.V.


  • Antibody alternative
  • Aspergillus niger spore
  • Colorimetric detection
  • Fungi sensor
  • M13 bacteriophage

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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