Abstract
Fungal spores are an important component of bio-aerosols and ubiquitous in indoor environments, leading to adverse health problems. Because concerns about indoor air quality have increased, rapid and sensitive detection methods are needed for personal and public health. In this study, we developed a sensitive colorimetric biosensor for detecting allergenic fungal spores using engineered M13 bacteriophages. Aspergillus niger spore-binding phages, showing high binding affinity to A. niger spores, were identified by phage display screening. The phages were biotinylated on the major coat protein pVIII for binding with streptavidin–HRP conjugates, resulting in enhanced color change. This engineered phage exhibited higher sensitivity and specificity for A. niger spores than conventionally used antibody-based detection systems.
Original language | English |
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Article number | 134244 |
Journal | Sensors and Actuators B: Chemical |
Volume | 393 |
DOIs | |
Publication status | Published - 2023 Oct 15 |
Bibliographical note
Publisher Copyright:© 2023 Elsevier B.V.
Keywords
- Antibody alternative
- Aspergillus niger spore
- Colorimetric detection
- Fungi sensor
- M13 bacteriophage
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Instrumentation
- Condensed Matter Physics
- Surfaces, Coatings and Films
- Metals and Alloys
- Electrical and Electronic Engineering
- Materials Chemistry