Engineered phage matrix stiffness-modulating osteogenic differentiation

Hee Sook Lee, Jeong In Kang, Woo Jae Chung, Do Hoon Lee, Byung Yang Lee, Seung Wuk Lee, So Young Yoo

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.

Original languageEnglish
Pages (from-to)4349-4358
Number of pages10
JournalACS Applied Materials and Interfaces
Volume10
Issue number5
DOIs
Publication statusPublished - 2018 Feb 7

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C1067) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03935221).

Publisher Copyright:
© 2018 American Chemical Society.

Keywords

  • differentiation
  • matrix
  • osteogenic
  • phage
  • stiffness

ASJC Scopus subject areas

  • General Materials Science

Fingerprint

Dive into the research topics of 'Engineered phage matrix stiffness-modulating osteogenic differentiation'. Together they form a unique fingerprint.

Cite this