Abstract
Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.
Original language | English |
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Pages (from-to) | 4349-4358 |
Number of pages | 10 |
Journal | ACS Applied Materials and Interfaces |
Volume | 10 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2018 Feb 7 |
Bibliographical note
Funding Information:This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C1067) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03935221).
Publisher Copyright:
© 2018 American Chemical Society.
Keywords
- differentiation
- matrix
- osteogenic
- phage
- stiffness
ASJC Scopus subject areas
- General Materials Science