Engineered phage matrix stiffness-modulating osteogenic differentiation

Hee Sook Lee; Jeong In Kang; Woo Jae Chung; Do Hoon Lee; Byung Yang Lee; Seung Wuk Lee; So Young Yoo

doi:10.1021/acsami.7b17871

Engineered phage matrix stiffness-modulating osteogenic differentiation

Hee Sook Lee
, Jeong In Kang
, Woo Jae Chung
, Do Hoon Lee
, Byung Yang Lee
, Seung Wuk Lee
, So Young Yoo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.

Original language	English
Pages (from-to)	4349-4358
Number of pages	10
Journal	ACS Applied Materials and Interfaces
Volume	10
Issue number	5
DOIs	https://doi.org/10.1021/acsami.7b17871
Publication status	Published - 2018 Feb 7

Bibliographical note

Funding Information:
This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C1067) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03935221).

Publisher Copyright:
© 2018 American Chemical Society.

Keywords

differentiation
matrix
osteogenic
phage
stiffness

ASJC Scopus subject areas

General Materials Science

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10.1021/acsami.7b17871

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abstract = "Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.",

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AU - Kang, Jeong In

AU - Chung, Woo Jae

AU - Lee, Do Hoon

AU - Lee, Byung Yang

AU - Lee, Seung Wuk

AU - Yoo, So Young

N1 - Funding Information: This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI16C1067) and by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03935221). Publisher Copyright: © 2018 American Chemical Society.

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N2 - Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.

AB - Herein, we demonstrate an engineered phage mediated matrix for osteogenic differentiation with controlled stiffness by cross-linking the engineered phage displaying Arg-Gly-Asp (RGD) and His-Pro-Gln (HPQ) with various concentrations of streptavidin or polymer, poly(diallyldimethylammonium)chloride (PDDA). Osteogenic gene expressions showed that they were specifically increased when MC3T3 cells were cultured on the stiffer phage matrix than the softer one. Our phage matrixes can be easily functionalized using chemical/genetic engineering and used as a stem cell tissue matrix stiffness platform for modulating differential cell expansion and differentiation.

KW - differentiation

KW - matrix

KW - osteogenic

KW - phage

KW - stiffness

UR - https://www.scopus.com/pages/publications/85041894832

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JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 5

ER -

Engineered phage matrix stiffness-modulating osteogenic differentiation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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