Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from CO2

Soyoung Kim; Yong Jae Jang; Gyeongtaek Gong; Sun Mi Lee; Youngsoon Um; Kyoung Heon Kim; Ja Kyong Ko

doi:10.1186/s12934-022-01962-7

Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from CO₂

Soyoung Kim, Yong Jae Jang, Gyeongtaek Gong, Sun Mi Lee, Youngsoon Um, Kyoung Heon Kim, Ja Kyong Ko

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Background: A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO₂) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as a model PHB producer, the PHB production rate from CO₂ is still too low for commercialization. Results: Here, we engineer the carbon fixation metabolism to improve CO₂ utilization and increase PHB production. We explore the possibilities to enhance the lithoautotrophic cell growth and PHB production by introducing additional copies of transcriptional regulators involved in Calvin Benson Bassham (CBB) cycle. Both cbbR and regA-overexpressing strains showed the positive phenotypes for 11% increased biomass accumulation and 28% increased PHB production. The transcriptional changes of key genes involved in CO₂—fixing metabolism and PHB production were investigated. Conclusions: The global transcriptional regulator RegA plays an important role in the regulation of carbon fixation and shows the possibility to improve autotrophic cell growth and PHB accumulation by increasing its expression level. This work represents another step forward in better understanding and improving the lithoautotrophic PHB production by C. necator H16.

Original language	English
Article number	231
Journal	Microbial Cell Factories
Volume	21
Issue number	1
DOIs	https://doi.org/10.1186/s12934-022-01962-7
Publication status	Published - 2022 Dec

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean government (MSIT) (No. RS-2022-00156236). The authors also appreciate the additional support provided by the Korea Institute of Science and Technology (KIST) Institutional Programs (2E31853 and 2E31833).

Publisher Copyright:
© 2022, The Author(s).

Keywords

Calvin Benson Bassham (CBB) cycle
Carbon dioxide fixation
Cupriavidus necator H16
Lithoautotrophic culture
Polyhydroxybutyrate (PHB)
Transcriptional regulator

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1186/s12934-022-01962-7

Cite this

@article{246066566cdb4e93b8ca3570c206553d,

title = "Engineering Cupriavidus necator H16 for enhanced lithoautotrophic poly(3-hydroxybutyrate) production from CO2",

abstract = "Background: A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO2) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as a model PHB producer, the PHB production rate from CO2 is still too low for commercialization. Results: Here, we engineer the carbon fixation metabolism to improve CO2 utilization and increase PHB production. We explore the possibilities to enhance the lithoautotrophic cell growth and PHB production by introducing additional copies of transcriptional regulators involved in Calvin Benson Bassham (CBB) cycle. Both cbbR and regA-overexpressing strains showed the positive phenotypes for 11% increased biomass accumulation and 28% increased PHB production. The transcriptional changes of key genes involved in CO2—fixing metabolism and PHB production were investigated. Conclusions: The global transcriptional regulator RegA plays an important role in the regulation of carbon fixation and shows the possibility to improve autotrophic cell growth and PHB accumulation by increasing its expression level. This work represents another step forward in better understanding and improving the lithoautotrophic PHB production by C. necator H16.",

keywords = "Calvin Benson Bassham (CBB) cycle, Carbon dioxide fixation, Cupriavidus necator H16, Lithoautotrophic culture, Polyhydroxybutyrate (PHB), Transcriptional regulator",

author = "Soyoung Kim and Jang, {Yong Jae} and Gyeongtaek Gong and Lee, {Sun Mi} and Youngsoon Um and Kim, {Kyoung Heon} and Ko, {Ja Kyong}",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean government (MSIT) (No. RS-2022-00156236). The authors also appreciate the additional support provided by the Korea Institute of Science and Technology (KIST) Institutional Programs (2E31853 and 2E31833). Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

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doi = "10.1186/s12934-022-01962-7",

language = "English",

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AU - Kim, Soyoung

AU - Jang, Yong Jae

AU - Gong, Gyeongtaek

AU - Lee, Sun Mi

AU - Um, Youngsoon

AU - Kim, Kyoung Heon

AU - Ko, Ja Kyong

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean government (MSIT) (No. RS-2022-00156236). The authors also appreciate the additional support provided by the Korea Institute of Science and Technology (KIST) Institutional Programs (2E31853 and 2E31833). Publisher Copyright: © 2022, The Author(s).

PY - 2022/12

Y1 - 2022/12

N2 - Background: A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO2) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as a model PHB producer, the PHB production rate from CO2 is still too low for commercialization. Results: Here, we engineer the carbon fixation metabolism to improve CO2 utilization and increase PHB production. We explore the possibilities to enhance the lithoautotrophic cell growth and PHB production by introducing additional copies of transcriptional regulators involved in Calvin Benson Bassham (CBB) cycle. Both cbbR and regA-overexpressing strains showed the positive phenotypes for 11% increased biomass accumulation and 28% increased PHB production. The transcriptional changes of key genes involved in CO2—fixing metabolism and PHB production were investigated. Conclusions: The global transcriptional regulator RegA plays an important role in the regulation of carbon fixation and shows the possibility to improve autotrophic cell growth and PHB accumulation by increasing its expression level. This work represents another step forward in better understanding and improving the lithoautotrophic PHB production by C. necator H16.

AB - Background: A representative hydrogen-oxidizing bacterium Cupriavidus necator H16 has attracted much attention as hosts to recycle carbon dioxide (CO2) into a biodegradable polymer, poly(R)-3-hydroxybutyrate (PHB). Although C. necator H16 has been used as a model PHB producer, the PHB production rate from CO2 is still too low for commercialization. Results: Here, we engineer the carbon fixation metabolism to improve CO2 utilization and increase PHB production. We explore the possibilities to enhance the lithoautotrophic cell growth and PHB production by introducing additional copies of transcriptional regulators involved in Calvin Benson Bassham (CBB) cycle. Both cbbR and regA-overexpressing strains showed the positive phenotypes for 11% increased biomass accumulation and 28% increased PHB production. The transcriptional changes of key genes involved in CO2—fixing metabolism and PHB production were investigated. Conclusions: The global transcriptional regulator RegA plays an important role in the regulation of carbon fixation and shows the possibility to improve autotrophic cell growth and PHB accumulation by increasing its expression level. This work represents another step forward in better understanding and improving the lithoautotrophic PHB production by C. necator H16.

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