Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae

Kyung Ok Yu; Seung Wook Kim; Sung Ok Han

doi:10.1016/j.biortech.2010.01.066

Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae

Kyung Ok Yu, Seung Wook Kim, Sung Ok Han

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4 g L^-1 ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.

Original language	English
Pages (from-to)	4157-4161
Number of pages	5
Journal	Bioresource technology
Volume	101
Issue number	11
DOIs	https://doi.org/10.1016/j.biortech.2010.01.066
Publication status	Published - 2010 Jun

Keywords

Ethanol
Fermentation
Glycerol
Saccharomyces cerevisiae

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2010.01.066

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title = "Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae",

abstract = "Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4 g L-1 ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.",

keywords = "Ethanol, Fermentation, Glycerol, Saccharomyces cerevisiae",

author = "Yu, {Kyung Ok} and Kim, {Seung Wook} and Han, {Sung Ok}",

note = "Funding Information: We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This research was supported by the core Environmental Technology Development project for Next Generation funded by the Ministry of Environment, Republic of Korea (No. 032-091-019 ).",

year = "2010",

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doi = "10.1016/j.biortech.2010.01.066",

language = "English",

volume = "101",

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journal = "Bioresource Technology",

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T1 - Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae

AU - Yu, Kyung Ok

AU - Kim, Seung Wook

AU - Han, Sung Ok

N1 - Funding Information: We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This research was supported by the core Environmental Technology Development project for Next Generation funded by the Ministry of Environment, Republic of Korea (No. 032-091-019 ).

PY - 2010/6

Y1 - 2010/6

N2 - Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4 g L-1 ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.

AB - Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4 g L-1 ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.

KW - Ethanol

KW - Fermentation

KW - Glycerol

KW - Saccharomyces cerevisiae

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JO - Bioresource Technology

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IS - 11

ER -

Engineering of glycerol utilization pathway for ethanol production by Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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