Abstract
Saccharomyces cerevisiae was metabolically engineered to improve ethanol production from glycerol. High rates of glycerol utilization were achieved by simultaneous overexpression of glycerol dehydrogenase (Gcy) and dihydroxyacetone kinase (Dak), which are the enzymes responsible for the conversion of glycerol to glycolytic intermediate dihydroxyacetone phosphate. As a result, ethanol production in YPH499 (pGcyaDak) was about 2.4-fold higher than wild strain. We have also successfully expressed a glycerol uptake protein (Gup1). The overall ethanol production in strain YPH499 (pGcyaDak, pGupCas) was 3.4-fold more than in wild strain, with about 2.4 g L-1 ethanol produced. These experimental results confirmed our metabolic pathway strategies which improve the production of ethanol.
| Original language | English |
|---|---|
| Pages (from-to) | 4157-4161 |
| Number of pages | 5 |
| Journal | Bioresource technology |
| Volume | 101 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2010 Jun |
Bibliographical note
Funding Information:We thank Roy H. Doi (University of California, Davis) for critical reading of the manuscript. This research was supported by the core Environmental Technology Development project for Next Generation funded by the Ministry of Environment, Republic of Korea (No. 032-091-019 ).
Keywords
- Ethanol
- Fermentation
- Glycerol
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal
