Enhanced accumulation of gamma-aminobutyric acid in rice bran using anaerobic incubation with various additives

Su Jin Oh, Hyun Soo Kim, Seung Taik Lim, Chagam Koteswara Reddy

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

An anaerobic incubation for the enzymatic production of gamma-aminobutyric acid (GABA) in rice bran with the addition of glutamate, hydrolyzed wheat protein (HWP), yeast extract (YE) and pyridoxal-5-phosphate (PLP) was investigated. Rice bran was moistened (30% moisture content) with an electrolyzed oxidizing water and anaerobically incubated under nitrogen at 40 °C for 8 h. The incubation activated the glutamate decarboxylase (GAD) in rice bran and increased the GABA content from 10.7 to 171.5 mg/100 g. The addition of glutamate and protein hydrolysates further amplified the GABA content in the treated rice bran: 974.9, 487.4, and 372.8 mg/100 g, with 2.25% glutamate, 6% HWP, and 8% YE, respectively. Furthermore, addition of PLP (1.48 mg/100 g) as a coenzyme for GAD, together with 2.25% glutamate addition, could raise the GABA accumulation in rice bran to 2242 mg/100 g. Other amino acids in rice bran were changed in their composition by the anaerobic treatment.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalFood Chemistry
Volume271
DOIs
Publication statusPublished - 2019 Jan 15

Bibliographical note

Funding Information:
This research was financially supported by Small and Medium Business Administration in Korea , South Korea (Grants No. R1626002 ). In addition, authors thank the BK21 PLUS program at Korea University for providing the support to carry out the research.

Funding Information:
This research was financially supported by Small and Medium Business Administration in Korea, South Korea (Grants No. R1626002). In addition, authors thank the BK21 PLUS program at Korea University for providing the support to carry out the research.

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

  • Anaerobic incubation
  • Gamma-aminobutyric acid
  • Hydrolyzed wheat protein
  • Pyridoxal-5-phosphate
  • Rice bran
  • Yeast extract

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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